Overview
- Presents a comprehensive collection of cutting-edge methods for research on hepatocytes and stem cells
- Supplies core methods for human hepatocyte isolation, culture, differentiation, cryopreservation, and transplantation
- Examines the state of the art of liver development
- Provides methods for generating hepatocytes from stem cells of different sources
- Details technical aspects of hepatocyte use in drug metabolism and toxicology as well as technical aspects of hepatocyte use in endogenous metabolism and virology
- Features chimeric mice with humanized liver and bioartificial liver support and liver biotherapy
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 640)
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Table of contents (29 protocols)
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Front Matter
Keywords
About this book
Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.
Reviews
From the reviews:
“Hepatocytes presents a well matched mix of theory and practice. … A very welcome general theme throughout the book are the detailed practical aspects. Some chapters might almost be training manuals in this respect. … I found the book very hard to criticise To me, it was the ideal textbook … . Moreover each chapter was written by contributors that dripped with experience. … the quality of the text is so outstanding.” (Graham Lappin, BTS Newsletter, Issue 37, Winter, 2010)
“Eighty authors have collaborated to construct 29 chapters … . the quality of the paper, the layout and print size, and the overall construction of the book are excellent. The 553 pages are abundantly illustrated and annotated with 1591 references followed by a 19-page index … . The work is a worthy and elegant addition to their ‘Methods in Molecular Biology’ series and would be equally at home, and useful, around the laboratory, on the office shelf or in a reference library.” (S. C. Mitchell, ISSX Newsletter, February, 2011)
Editors and Affiliations
Bibliographic Information
Book Title: Hepatocytes
Book Subtitle: Methods and Protocols
Editors: Patrick Maurel
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-688-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-687-0Published: 07 May 2010
Softcover ISBN: 978-1-4939-5716-3Published: 23 August 2016
eBook ISBN: 978-1-60761-688-7Published: 20 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIX, 553
Topics: Human Physiology, Molecular Medicine, Hepatology