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Biomedical Sciences - Human Genetics | DNA Cloning and Assembly Methods

DNA Cloning and Assembly Methods

Series: Methods in Molecular Biology, Vol. 1116

Valla, Svein, Lale, Rahmi (Eds.)

2014, XI, 303 p. 74 illus., 50 illus. in color.

A product of Humana Press
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  • Included methods based on mechanism
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.

With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide  scientist with a valuable and useful resource for wet lab researchers within life sciences.

Content Level » Professional/practitioner

Keywords » DNA cloning - DNA fragments - II restriction enzymes - IIS restriction enzymes - cloning - genes - protein expression - sequence overlap - single stranded annealing

Related subjects » Human Genetics

Table of contents 

1. BioBrick Assembly Standards and Techniques and Associated Software Tools

Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås, and Rahmi Lale


2. Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning

Ryan E. Hill, Julian J. Eaton-Rye


3. Quick and Clean Cloning

Frank Thieme and Sylvestre Marillonnet


4. Hierarchical Ligation-Independent Assembly of PCR Fragments

Jonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson


5. USER-Derived Cloning Methods and Their Primer Design

Bo Salomonsen, Uffe H Mortensen, Barbara A Halkier


6. Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly

Yoav Peleg and Tamar Unger


7. A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform

Ariel Erijman, Julia M. Shifman, and Yoav Peleg


8. Circular Polymerase Extension Cloning

Jiayuan Quan and Jingdong Tian


9. Golden Gate Cloning

Carola Engler and Sylvestre Marillonnet


10. Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell, Diego Orzaez


11. FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression

Eric R. Geertsma


12. Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing

Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada


13. Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR

Chun You and  Y.-H. Percival Zhang


14. Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination

Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter, and Dominic Esposito


15. Application of In-Fusion™ Cloning for the Parallel Construction of Escherichia coli Expression Vectors
Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert, Raymond J. Owens


16. SeamLess Ligation Cloning Extract(SLiCE)Cloning Method

Yongwei Zhang, Uwe Werling, and Winfried Edelmann


17. j5 DNA Assembly Design Automation

Nathan J. Hillson


18. FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis

Ruslan Kalendar, David Lee, and Alan H. Schulman

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