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Details methods that are used to study DNA using electrophoresis
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Content Level »Professional/practitioner
Keywords »2-dimentional gel electrophoresis - DNA electrophoresis - DNA molecules - PCR products - Pulse Field Gel Electrophoresis - agarose gels - bacterial chromosomes - dsDNA molecules - electrophoreses - eukaryotic chromosomes - fluorescence in situ hybrifization - linear DNA - plasmid digests - protein-DNA interactions - ssDNA