Kolpashchikov, Dmitry M., Gerasimova, Yulia V. (Eds.)
2013, XIV, 320 p. 85 illus., 58 illus. in color.
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Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Content Level »Professional/practitioner
Keywords »DNA - DNA for amplification and analysis - RNA - RNA monitoring in live cells - capillary electrophoresis - fluorescent in situ hybridization - isothermal amplification techniques - microarrays - molecular beacon probes for micro RNA analysis - nucleic acid detection methods - point-of care diagnostics - qPCR chemistries - signal amplification technique - visual or electric signals