Overview
- Presents current up-to-date methodologies
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1024)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
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Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Circulating MicroRNAs
Book Subtitle: Methods and Protocols
Editors: Nobuyoshi Kosaka
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-453-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-452-4Published: 30 May 2013
Softcover ISBN: 978-1-4939-6299-0Published: 23 August 2016
eBook ISBN: 978-1-62703-453-1Published: 30 May 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 270
Number of Illustrations: 22 b/w illustrations, 22 illustrations in colour
Topics: Human Genetics