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Biomedical Sciences - Human Genetics | Tandem Repeats in Genes, Proteins, and Disease - Methods and Protocols

Tandem Repeats in Genes, Proteins, and Disease

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1017

Hatters, Danny M., Hannan, Anthony J. (Eds.)

2013, XI, 258 p. 55 illus., 23 illus. in color.

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  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences.  Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine.  Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states.  This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular  and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.

Content Level » Professional/practitioner

Keywords » DNA sequences - animal models of tandem - cellular approaches - codons - human disorders - poly-amino acid sequences - tandem repeats in DNA - tandem repeats in RNA

Related subjects » Human Genetics

Table of contents 

1. Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins

Andrey S. Tsvetkov, D. Michael Ando & Steven Finkbeiner

 

2. Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces

Kathleen A. Burke and Justin Legleiter

 

3. Morphometric Analysis of Huntington’s Disease Neurodegeneration in Drosophila

Wan Song, Marianne R. Smith, Adeela Syed, Tamas Lukacsovich, Brett A. Barbaro, Judith Purcell, Doug J. Bornemann, John Burke and J. Lawrence Marsh

 4. Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical UltracentrifugeSaskia Polling, Danny M. Hatters, and Yee-Foong Mok

 

5. A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins

Amy L. Robertson and Stephen P. Bottomley

 

6. Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation

Yasmin M. Ramdzan, Rebecca Wood, Danny M. Hatters

 

7. Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders

Emma L. Burrows and Anthony J. Hannan

 

8. PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example

Tan M.H., Gécz J., Shoubridge C.

 

9. Challenges of “sticky” Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions

Mattiske T.R., Tan M.H., Gécz J. and Shoubridge C.

 

10. Molecular Pathology of Polyalanine Expansion Disorders-new
Perspectives from Mouse Models

James N Hughes and Paul Q Thomas

 

11. Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation

Martin L. Duennwald

 

12. Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples

Gregor P. Lotz and Andreas Weiss

 

13. Modeling and Analysis of Repeat RNA Toxicity in Drosophila

Samaraweera, S.E. , O’Keefe, L.V. , van Eyk, C.L. , Lawlor, K.T., Humphreys D.T., Suter, C.M. and Richards, R.I.

 

14. Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis

Mats Holmberg, Ellen A.A. Nollen

 

15. Kinetic Analysis of Aggregation Data

Regina M. Murphy

 

16. A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins

Eric D. Ross, Kyle S. MacLea, Charles Anderson, and Asa Ben-Hur

 

17. Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP

Patrick Lajoie and Erik Lee Snapp

 

18. Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism

Lori J. Cooper and Diane E. Merry

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