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Biomedical Sciences - Human Genetics | Microsatellites - Methods and Protocols

Microsatellites

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1006

Kantartzi, Stella K. (Ed.)

2013, XI, 339 p. 71 illus., 43 illus. in color.

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  • Includes cutting-edge methods and protocols for microsatellite markers in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems.  Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research.  Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.

Content Level » Professional/practitioner

Keywords » Capillary sequencers - Fragment analysis - Genotyping - Microsatellite markers - Molecular markers - SSRs - Simple sequence repeats

Related subjects » Human Genetics

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