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Biomedical Sciences - Human Genetics | Biolistic DNA Delivery - Methods and Protocols

Biolistic DNA Delivery

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 940

Sudowe, Stephan, Reske-Kunz, Angelika B. (Eds.)

2013, XIV, 415 p. 80 illus., 33 illus. in color.

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  • Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells
  • Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field
  • Features key tips and implementation advice to guarantee successful results

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.  Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

All-inclusive and cutting-edge,  Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

Content Level » Professional/practitioner

Keywords » Artificial DNA introduction - Biolistic transfection - DNA vaccination - Gene guns - Gene transfer

Related subjects » Biotechnology - Human Genetics

Table of contents 

Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

 

1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun

            Caixia Gao and Klaus K. Nielsen

 

2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery

            Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky

 

3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues

            Mulpuri Sujatha and K.B.R.S. Visarada

 

4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)

            Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen

 

5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies

            Victor Gaba, Moshe Lapidot, and Amit Gal-On

 

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun

            Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

 

Part II: Biolistic DNA Delivery in Nematodes

 

7. Biolistic Transformation of Caenorhabditis elegans

            Meltem Isik and Eugene Berezikov

 

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans

            Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

 

9. Biolistic Transformation of Brugia malayi

            Tarig B. Higazi and Thomas R. Unnasch

 

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

 

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells

            Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

 

11. Biolistic Transfection of Tumor Tissue Samples

            Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

 

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes

            David F. Steele, Ying Dou, and David Fedida

 

13. Biolistic Transfection of Neurons in Organotypic Brain Slices

            John A. O’Brien and Sarah C.R. Lummis

 

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo

 

14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

            Meng-Chi Yen and Ming-Derg Lai

 

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery

            Ahmad Jalili

 

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs

            Steve Pascolo

 

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter

            Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

 

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice

            Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

 

Part V: Biolistic DNA Vaccination in Animal Disease Models

 

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets

            Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

 

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models

            Gustaf Ahlén, Matti Sällberg, and Lars Frelin

 

21. Gene Gun Immunization to Combat Malaria

            Elke S. Bergmann-Leitner and Wolfgang W. Leitner

 

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination

            Toshi Nagata and Yukio Koide

 

23. Biolistic DNA Vaccination Against Trypanosoma Infection

            Marianne Bryan, Siobhan Guyach, and Karen A. Norris

 

24. Biolistic DNA Vaccination Against Melanoma

            Julia Steitz and Thomas Tüting

 

25. Biolistic DNA Vaccination Against Cervical Cancer

            Michal Šmahel

 

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy

            Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

 

27. Safety Assessment of Biolistic DNA Vaccination

            Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

 

Part VI: Related Applications for Biolistic Delivery of Molecules

 

28. DiOlistics: Delivery of Fluorescent Dyes into Cells

            Nyssa Sherazee and Veronica A. Alvarez

 

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)

            Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

 

14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

            Meng-Chi Yen and Ming-Derg Lai

 

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery

            Ahmad Jalili

 

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs

            Steve Pascolo

 

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter

            Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

 

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice

            Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

 

Part V: Biolistic DNA Vaccination in Animal Disease Models

 

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets

            Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

 

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models

            Gustaf Ahlén, Matti Sällberg, and Lars Frelin

 

21. Gene Gun Immunization to Combat Malaria

            Elke S. Bergmann-Leitner and Wolfgang W. Leitner

 

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination

            Toshi Nagata and Yukio Koide

 

23. Biolistic DNA Vaccination Against Trypanosoma Infection

            Marianne Bryan, Siobhan Guyach, and Karen A. Norris

 

24. Biolistic DNA Vaccination Against Melanoma

            Julia Steitz and Thomas Tüting

 

25. Biolistic DNA Vaccination Against Cervical Cancer

            Michal Šmahel

 

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy

            Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

 

27. Safety Assessment of Biolistic DNA Vaccination

            Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

 

Part VI: Related Applications for Biolistic Delivery of Molecules

 

28. DiOlistics: Delivery of Fluorescent Dyes into Cells

            Nyssa Sherazee and Veronica A. Alvarez

 

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)

            Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

            Mulpuri Sujatha and K.B.R.S. Visarada

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