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Collects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field
Covers current and emerging methods for production of viral and non-viral gene transfer vectors
Provides detailed protocols for applications in stem cell biology, cancer research and infectious disease
In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
Table of Contents 1 Preparation and Quantification of Pseudotyped Retroviral Vector Hong Yu and Young Jik Kwon 2 Production of Retroviral Vectors for Clinical Use Kenneth Cornetta, Lilith Reeves and Scott Cross 3 Methods for the Production of Helper-Dependent Adenoviral Vectors Donna J. Palmer and Philip Ng 4 Methods for the Production of First Generation Adenoviral Vectors Donna J. Palmer and Philip Ng 5 Large Scale Production of Recombinant Adeno-Associated Vectors Alejandro Negrete and Robert M. Kotin 6 Construction and Production of Recombinant Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, James B. Wechuck, Shaohua Huang and Joseph C. Glorioso 7 Plasmid-based Gene Transfer in Mouse Skeletal Muscle by Electroporation Johnathan D. Schertzer and Gordon S. Lynch 8 Chitosan Nanoparticle–Mediated Gene Transfer Dongwon Lee and Shyam S. Mohapatra 9 PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues Gordon J. Lutz, Shashank R. Sirsi and Jason H. Williams 10 Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes Kai K. Ewert, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans and Cyrus R. Safinya 11 Applications of Lentiviral Vectors in Non-invasive Molecular Imaging Abhijit De, Shahriar Shah Yaghoubi and Sanjiv Sam Gambhir 12 Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A Christopher B. Doering 13 Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells Lucienne M. Ide, Elisabeth Javazon and H. Trent Spencer 14 Assessment of CFTR Function After Gene Transfer in Vitro and in Vivo Uta Griesenbach,Felix M. Munkonge, Stephanie Sumner-Jones, Emma Holder, Stephen N. Smith, A. Christopher Boyd, Deborah R. Gill, Stephen C. Hyde, David Porteous and Eric WFW Alton 15 Oncolytic Adenoviruses for Cancer Gene Therapy Ta-Chiang Liu, Stephen H. Thorne and David H. Kim 16 Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy Yi Lai, Dejia Li, Yongping Yue and Dongsheng Duan 17 Prevention of Type One Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells Jessamyn Bagley, Chaorui Tian and John Iacomini 18 Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells Mingjie Li and John J. Rossi 19 Nanoparticle-Mediated Gene Delivery to the Lung Rajagopal Ramesh 20 Retroviral-mediated Gene Therapy for the Differe
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