Editors:
- Includes cutting-edge methods and protocols for the study of microbial gene essentiality
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1279)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
About this book
This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.
Editors and Affiliations
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Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
Long Jason Lu
Bibliographic Information
Book Title: Gene Essentiality
Book Subtitle: Methods and Protocols
Editors: Long Jason Lu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2398-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2397-7Published: 31 January 2015
Softcover ISBN: 978-1-4939-4862-8Published: 05 October 2016
eBook ISBN: 978-1-4939-2398-4Published: 30 January 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 248
Number of Illustrations: 27 b/w illustrations, 15 illustrations in colour