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  • © 2015

PCR Primer Design

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1275)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
  2. Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes

    • Reidar Andreson, Lauris Kaplinski, Maido Remm
    Pages 1-16
  3. Design of Primers and Probes for Quantitative Real-Time PCR Methods

    • Alicia Rodríguez, Mar Rodríguez, Juan J. Córdoba, María J. Andrade
    Pages 31-56
  4. Degenerate Primer Design for Highly Variable Genomes

    • Kelvin Li, Susmita Shrivastava, Timothy B. Stockwell
    Pages 103-115
  5. Allele-Specific Real-Time Polymerase Chain Reaction as a Tool for Urate Transporter 1 Mutation Detection

    • Juliet O. Makanga, Antonius Christianto, Tetsuya Inazu
    Pages 117-125
  6. MultiPLX: Automatic Grouping and Evaluation of PCR Primers

    • Lauris Kaplinski, Maido Remm
    Pages 127-142
  7. In Silico PCR Primer Designing and Validation

    • Anil Kumar, Nikita Chordia
    Pages 143-151
  8. Primer Design Using Primer Express® for SYBR Green-Based Quantitative PCR

    • Amarjeet Singh, Girdhar K. Pandey
    Pages 153-164
  9. Designing Primers for SNaPshot Technique

    • Greiciane Gaburro Paneto, Francisco de Paula Careta
    Pages 165-172
  10. Rapid and Simple Method of qPCR Primer Design

    • Brenda Thornton, Chhandak Basu
    Pages 173-179
  11. PRIMEGENSw3: A Web-Based Tool for High-Throughput Primer and Probe Design

    • Garima Kushwaha, Gyan Prakash Srivastava, Dong Xu
    Pages 181-199
  12. Selecting Specific PCR Primers with MFEprimer

    • Wubin Qu, Chenggang Zhang
    Pages 201-213
  13. Back Matter

    Pages 215-216

About this book

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Editors and Affiliations

  • Department of Biology, California State University, Northridge, Los Angeles, USA

    Chhandak Basu

Bibliographic Information

  • Book Title: PCR Primer Design

  • Editors: Chhandak Basu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2365-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2364-9Published: 19 February 2015

  • Softcover ISBN: 978-1-4939-4755-3Published: 08 October 2016

  • eBook ISBN: 978-1-4939-2365-6Published: 19 February 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 216

  • Number of Illustrations: 28 b/w illustrations, 45 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access