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Biomedical Sciences - Human Genetics | Regulatory Non-Coding RNAs - Methods and Protocols

Regulatory Non-Coding RNAs

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1206

Carmichael, Gordon G. (Ed.)

2015, X, 181 p. 39 illus., 10 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols on non-coding RNAs
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs, and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

Content Level » Professional/practitioner

Keywords » Fluorescence in situ hybridization - Long non-coding RNAs - MicroRNA targets - Non-coding transcriptome - Protein-RNA interactions - RNA-binding proteins - siRNAs

Related subjects » Human Genetics

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