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Biomedical Sciences - Human Genetics | Animal Endo-SiRNAs - Methods and Protocols

Animal Endo-SiRNAs

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1173

Werner, Andreas (Ed.)

2014, X, 182 p. 36 illus., 24 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.  These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.


Content Level » Professional/practitioner

Keywords » C. elegans - Dicer molecule - Short interfering RNAs (siRNAs) - endo-siRNAs - gene expression - model systems - short RNAs - small RNAs - vertebrates

Related subjects » Human Genetics

Table of contents 

1. Targeted Small Non-coding RNA Directed Gene Activation in Human Cells

Caio Damski and Kevin V. Morris


2. Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs

Oliver Meikar and Noora Kotaja


3. Generation of Endo-siRNAs in Xenopus laevis Oocytes

Sammer Alnumeir and Andreas Werner


4. Analysis of Endo-siRNAs in Drosophila

Katharina Elmer, Stephanie Helfer , Milijana Mirkovic-Hösle and Klaus Förstemann


5. Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

Do-Hwan Lim, Chun-Taek Oh, Sung-Jun Han, and Young Sik Lee


6. Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans

Maria C. Ow, Nelson C. Lau, and Sarah E. Hall


7. Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs

Philip K. Shiu, Jimmy J. Zhuang, and Craig P. Hunter


8. Extraction and Non-radioactive Detection of Small RNA Molecules

Mark Carlile and Andreas Werner


9. p19 Mediated Enrichment and Detection of siRNAs

Jingmin Jin, Larry A. McReynolds, and Monika Gullerova


10. Detection of Small non-coding RNAs by in situ Hybridization using Probes of 2’-O-Methyl RNA + LNA

Martin Jensen Søe, Martin Dufva, and Kim Holmstrøm


11. Enhanced Detection of Small RNAs using a non-Radioactive Approach

Teresa T. Liu, Zhihua Li, and  Bino John


12. Computing siRNA and piRNA Overlap Signatures

Christophe Antoniewski


13. Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference

Marius van den Beek, Christophe Antoniewski and Clément Carré


14. Computational Analysis, Biochemical Purification, and Detection of tRNA-derived Small RNA Fragments

Simon P. Keam, Andrew Sobala, David T. Humphreys, Catherine M. Suter, and Gyorgy Hutvagner

15. Differential DNA Methylation Patters in Endo-siRNA Mediated Silencing of LINE-1 Retrotranspons

Long Chen, Jane E Dahlstrom, and Danny Rangasamy

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