Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1121)
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Table of contents (30 protocols)
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Front Matter
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Basic Concepts and Enhancement of DNA Transfer via Electroporation
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Front Matter
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SiRNA Electrotransfer
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Front Matter
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Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues
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Front Matter
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About this book
Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
Editors and Affiliations
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The University of Texas M.D. Anderson Cancer Center, Houston, USA
Shulin Li, Jeffry Cutrera
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Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, USA
Richard Heller
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IPBS CNR, Toulouse, France
Justin Teissie
Bibliographic Information
Book Title: Electroporation Protocols
Book Subtitle: Preclinical and Clinical Gene Medicine
Editors: Shulin Li, Jeffry Cutrera, Richard Heller, Justin Teissie
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4614-9632-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4614-9631-1Published: 08 February 2014
Softcover ISBN: 978-1-4939-5492-6Published: 27 August 2016
eBook ISBN: 978-1-4614-9632-8Published: 08 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 349
Number of Illustrations: 20 b/w illustrations, 49 illustrations in colour
Topics: Human Genetics, Gene Expression