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Pancreatic Cancer

Methods and Protocols

Editors:

  • Details a broad range of protocols for molecular, cellular, pathological, and statistical analyses for pancreatic cancer
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 980)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Identification and Analysis of Precursors to Invasive Pancreatic Cancer

    • Hanno Matthaei, Marco Dal Molin, Anirban Maitra
    Pages 1-12
  3. Tissue Microarrays: Construction and Use

    • Helen Remotti
    Pages 13-28
  4. Immunohistochemistry of Pancreatic Neoplasia

    • Sukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar, Surinder K. Batra
    Pages 29-42
  5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications

    • Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck et al.
    Pages 61-120
  6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis

    • Alvin Makohon-Moore, Christine A. Iacobuzio-Donahue
    Pages 121-129
  7. DNA Methylation Analysis in Human Cancer

    • Eileen O’Sullivan, Michael Goggins
    Pages 131-156
  8. Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

    • Dan Laheru, Barbara Biedrzycki, Elizabeth M. Jaffee
    Pages 175-203
  9. The Prevention and Genetics of Pancreatic Cancer: A Programmatic Approach

    • Aimee L. Lucas, Michelle M. Chang, Marla D. Lipsyc, Harold Frucht
    Pages 205-214
  10. Development of Orthotopic Pancreatic Tumor Mouse Models

    • Wanglong Qiu, Gloria H. Su
    Pages 215-223
  11. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems

    • Emman Mascariñas, Guido Eibl, Paul J. Grippo
    Pages 225-248
  12. Quantification of Murine Pancreatic Tumors by High-Resolution Ultrasound

    • Stephen A. Sastra, Kenneth P. Olive
    Pages 249-266
  13. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells

    • Yuliya Pylayeva-Gupta, Kyoung Eun Lee, Dafna Bar-Sagi
    Pages 267-279
  14. Sphere-Forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

    • Yue J. Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach
    Pages 281-290
  15. Analysis of Transplanted Bone Marrow-Derived Cells in Chronic Pancreatitis

    • Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang
    Pages 291-300
  16. Analysis of Tumor-Associated Mucin Glycotopes by Western Transfer Methods

    • Vinayaga S. Gnanapragassam, Maneesh Jain, Surinder K. Batra
    Pages 331-340

About this book

Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA.  Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes.  The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer.   It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer. 

Reviews

From the reviews of the second edition:

“This book describes methods and techniques that are used in pancreatic cancer research. … It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. … It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an existing technique might be altered slightly to suit another need.” (Naomi Akagi, Doody’s Book Reviews, September, 2013)

Editors and Affiliations

  • , Irving Cancer Research Center, Columbia University, New York, USA

    Gloria H. Su

Bibliographic Information

  • Book Title: Pancreatic Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Gloria H. Su

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-287-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-286-5Published: 31 January 2013

  • Softcover ISBN: 978-1-4939-5982-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-287-2Published: 30 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 389

  • Topics: Cancer Research, Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access