Logo - springer
Slogan - springer

Biomedical Sciences - Cancer Research | Techniques and Methodological Approaches in Breast Cancer Research

Techniques and Methodological Approaches in Breast Cancer Research

Russo, Jose, Russo, Irma H.

2014, XVI, 287 p. 192 illus., 167 illus. in color.

Available Formats:

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.


(net) price for USA

ISBN 978-1-4939-0718-2

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase

learn more about Springer eBooks

add to marked items


Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.


(net) price for USA

ISBN 978-1-4939-0717-5

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days

add to marked items

  • Explores various cutting-edge methodologies used in breast cancer research, including stem cell separation, molecular analysis of gene function and more
  • Explores imaging techniques that are integral for studying the physiopathology of breast cancer
  • Authors are well known in the field of breast cancer

This book provides a detailed description of the most common techniques used in breast cancer research, with a special emphasis on the study of the normal breast as the basis for understanding the pathological processes in this organ.

The authors have poured their experience in the field of breast cancer research into this book, and described the process of handling and interpreting the complex organ that is the human breast using original data and unique material collected over the years.  They have established the concept of normality of the human breast and the morphological parameters to be used for distinguishing the normal from the diseased breast. The quality of the breast tissue obtained from autopsy material vs. core biopsies are carefully detailed and discussed, showing the morphological differences among the specimens according to their collection site. The use of laser capture microdissection and tissue microarray is described in detail and discussed with special references to the problems faced when the normal breast is under study. A chapter dedicated to the use of breast cancer cell lines is crafted around the importance of each cell line and its particularities for in vitro studies. In addition, the authors explore the new concept that cancer originates from two main cell types, basal and luminal cells . Particular attention is given to the study of the phenotypes commonly used for determining cell transformation, either spontaneously or by induced chemical agents. A companion chapter using in vivo models provides the adequate tools for those interested in studying the early events of cancer initiation, progression and metastasis. Special emphasis is given to the analysis of cells either by Flow cytometry and immuno-fluorescence techniques and the great potential of the use of in situ hybridization to study RNA transcripts. The book also features an original analysis of the putative markers of stem cells in breast cancer using immuno-cytochemical techniques, their interpretation and correlation with the prognosis of the disease. The last two chapters of the book are engaged in the utilization of RNA and DNA sequencing techniques and their values in understanding the physiological differences in the human breast, as well as the utilization of epigenetic methodologies in the understanding of the normal human breast.

The authors have established the methodological basis for studying the human breast in its normal and pathological conditions providing a unique set of tools and valuable references for researchers pursuing a deeper understanding of the human breast.

Content Level » Research

Keywords » breast cancer - epigenetics - proteomics - stem cells - transcriptome

Related subjects » Biochemistry & Biophysics - Biomedical Sciences - Cancer Research - Human Genetics - Molecular Medicine

Table of contents / Preface / Sample pages 

Popular Content within this publication 



Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Cancer Research.