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Cancer Vaccines

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Michael J.P. Lawman

    1. Morphogenesis, Inc., Tampa, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Patricia D. Lawman

    1. Morphogenesis, Inc., Tampa, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols to support the creation of future safe and efficacious cancer vaccines
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1139)

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Table of contents (40 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Manipulation and Modification of Immune Cells: Dendritic Cells

    1. Front Matter

      Pages 1-1
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    2. Single-Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules

      • Daphné Benteyn, An M. T. Van Nuffel, Sofie Wilgenhof, Aude Bonehill
      Pages 3-15

    3. Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine

      • Hyun-Ju Lee, Nu-Ri Choi, Manh-Cuong Vo, My-Dung Hoang, Youn-Kyung Lee, Je-Jung Lee
      Pages 17-26

    4. Pulsing Dendritic Cells with Whole Tumor Cell Lysates

      • Laura Alaniz, Manglio M. Rizzo, Guillermo Mazzolini
      Pages 27-31

    5. Antigen Trapping by Dendritic Cells for Antitumor Therapy

      • Chiranjib Pal
      Pages 33-40

    6. Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens

      • Manglio M. Rizzo, Laura Alaniz, Guillermo Mazzolini
      Pages 41-44

    7. Tumor Antigen-/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma

      • Sumit K. Hira, Deepak Verma, Partha P. Manna
      Pages 45-56

    8. Dendritic Cells Primed with Protein–Protein Fusion Adjuvant

      • Liying Wang, Yongli Yu
      Pages 57-75

    9. Antigen-Specific mRNA Transfection of Autologous Dendritic Cells

      • Fabian Benencia
      Pages 77-86

    10. Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material

      • Francesca Milano, K. K. Krishnadath
      Pages 87-95

    11. Dendritic Cells Transfected with Adenoviral Vectors as Vaccines

      • Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, James E. Talmadge
      Pages 97-118

    12. Genetic Modification of Dendritic Cells with RNAi

      • Xiao-Tong Song
      Pages 119-130

    13. Fast Monocyte-Derived Dendritic Cell-Based Immunotherapy

      • Gamal Ramadan
      Pages 131-144

    14. Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablation

      • Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, Yutaka Kawakami
      Pages 145-153

    15. Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines

      • Roeland Lameris, Famke L. Schneiders, Tanja D. de Gruijl, Hans J. van der Vliet
      Pages 155-165

  3. Manipulation and Modification of Immune Cells: T Lymphocytes and NK Cells

    1. Front Matter

      Pages 167-167
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    2. Modification of T Lymphocytes to Express Tumor Antigens

      • Aaron E. Foster, Xiao-Tong Song
      Pages 169-176

    3. Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor

      • Liza B. John, Tess M. Chee, David E. Gilham, Phillip K. Darcy
      Pages 177-187

    4. Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors

      • Serena K. Perna, Barbara Savoldo, Gianpietro Dotti
      Pages 189-200
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Keywords

About this book

Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future.

Reviews

From the reviews:

“This book describes cancer vaccine protocols targeting cancers that have already developed, stimulating the immune system against the tumor to stop cancer cell growth and reduce tumor burden. … The audience includes established researchers, professors, and healthcare professionals who have a basic understanding of general and tumor immunology. … This is a well-written, well-structured book that covers a rapidly developing and highly important field of vaccine cancer research.” (K. K. Wong, Doody’s Book Reviews, June, 2014)

Editors and Affiliations

  • Morphogenesis, Inc., Tampa, USA

    Michael J.P. Lawman, Patricia D. Lawman

Bibliographic Information

  • Book Title: Cancer Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Michael J.P. Lawman, Patricia D. Lawman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0345-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0344-3Published: 12 March 2014

  • Softcover ISBN: 978-1-4939-5195-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0345-0Published: 11 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 569

  • Number of Illustrations: 38 b/w illustrations, 42 illustrations in colour

  • Topics: Cancer Research, Oncology, Vaccine

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