Cell and organ printing has become a hot topic of scientific pursuit. Since several early publications between 2000-2003 that demonstrated proof-of-concept, cell and organ printing has blossomed into a rich area for scientific exploration that is being performed by researchers across the globe. Research has thoroughly demonstrated that living cells can be printed via a number of actuations including electrospray, extrusion via micropens and ejection through photothermal, thermal or optical mechanisms. This topic has come of age and it is ripe for exploring the underpinnings of the research to date. We have included research that uses printing technology to deposit or guide cells for tissue engineering applications and for completeness, we have also included chapters describing bacteria printing, biomolecular printing that could be used to build growth factors or recruitment macromolecules into scaffolds, tissue microdissection, as well as live cell printing. The breadth of approaches includes 3D freeform fabrication, ink jet, laser guidance and modified laser direct write techniques. We hope that this book is not the final word but the first word, defining how these tools have been used to take the first steps towards the ultimate goal of creating heterogeneous tissue constructs. Only time will tell whether cell printers will truly become organ printers, but the technologies described in this book hold promise to achieve what the field of regenerative medicine requires - functional 3D scaffolds with multiple cell types differentiated into functional tissue!
Forward, Bradley R. Ringeisen; BIOLOGICAL FREEFORM FABRICATION: Chapter 1: 3D On-Demand Bio-printing for the Creation of Engineered Tissues, Seung-Schik Yoo1 and Samuel Polio; INK JET APPROACHES: Chapter 2: Reconstruction of Biological Three-Dimensional Tissues: Bioprinting and Biofabrication using Inkjet Technology, Makoto Nakamura; Chapter 3: Piezoelectric inkjet printing of cells and biomaterials, Rachel Saunders, Julie Gough and Brian Derby; MODIFIED LASER INDUCED FORWARD TRANSFER (LIFT) APPROACHES: Chapter 4: Laser-Induced Forward Transfer: A Laser-Based Technique for Biomolecules Printing, P. Serra, M. Duocastella, J.M. Fernández-Pradas, J.L. Morenza; Chapter 5: Biological Laser Printing (BioLP) for High Resolution Cell Deposition, B.R. Ringeisen, C.M. Othon, Xingjia Wu, D.B. Krizman, M.M. Darfler, J.J. Anders, P.K. Wu; Chapter 6: High-Throughput Biological Laser Printing: Droplet Ejection Mechanism, Integration of a Dedicated Workstation, and Bioprinting of Cells and Biomaterials, Fabien Guillemot , Bertrand Guillotin , Sylvain Catros , Agnès Souquet , Candice Mezel , Virginie Keriquel , Ludovic Hallo, Jean-Christophe Frican , and Joëlle Amedee; Chapter 7: Absorbing-Film Assisted Laser Induced Forward Transfer of Sensitive Biological Subjects, B. Hopp, T. Smausz, A. Nógrádi; LASER GUIDANCE APPROACHES: Chapter 8: Laser Guidance-based Cell Micropatterning, Zhen Ma, Russell K. Pirlo, Julie X. Yun, Xiang Peng, and Bruce Z. Gao; SELF ORGANIZATION AND BIOLOGICAL GUIDANCE: Chapter 9: Emerging principles to rationally design tissues prone to self-organization, N.C. Rivron, J. Rouwkema, R. Truckenmüller, C.A. van Blitterswijk; Chapter 10: Biological Guidance, Jan-Thorsten Schantz, Harvey Chim; Chapter 11: Patterning Cells on Complex Curved Surface by Precision Spraying of Polymers, Mauris N. DeSilva ; Chapter 12: Fabrication of Growth Factor Array Using an Inkjet Printer, Kohei Watanabe, Tomoyo Fujiyama, Rina Mitsutake, Masaya Watanabe, Yukiko Tazaki, TakeshiMiyazaki, Ryoichi Matsuda; 3-DIMENSIONAL SCAFFOLD CELL PRINTING: Chapter 13: 3D-fiber deposition for tissue engineering and organ printing applications, N.E. Fedorovich, L. Moroni, J. Malda, J. Alblas, C.A. van Blitterswijk, W.J.A. Dhert; PRINTING BACTERIA: Chapter 14: Bacterial Cell Printing, Bradley R. Ringeisen, Lisa A. Fitzgerald, Stephen E. Lizewski, Justin C. Biffinger, Peter K. Wu; Index