Cells and Culture

1. Front Matter

   Pages i-liv

   PDF

2. Single-Cell Analysis and Engineering

   1. Front Matter

      Pages 1-1

      PDF

   2. Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells

      • Trissa Borgschulte, Fanny Delegrange, Christoph L. Bausch, David L. Hacker, Daniel W. Allison, Matthew V. Caple et al.

      Pages 3-8

   3. RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses

      • Peter de Haan, Alfred Engel, Walter de Vries, Jeroen van der Velden, Gerrit-Jan van Holst, Eddy Arnoldussen

      Pages 9-12

   4. Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in a 3-D Scaffold for Tissue Engineering

      • Eva-Maria Engelhardt, A. Oberbek, D. Aibibu, G. Backliwal, M. Adam, J. Hilborn et al.

      Pages 13-18

   5. Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element

      • Sarah Wulhfard, Stéphanie Tissot, David L. Hacker, Florian M. Wurm

      Pages 19-23

   6. Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production

      • Josef Schulze-Horsel, Mareike Schulze, Yvonne Genzel, Udo Reichl

      Pages 25-28

   7. Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation

      • Christian Weber, Stephanie Gokorsch, Peter Czermak

      Pages 29-34

   8. Cell Xpress™-Assisted Analysis of Clone Stability in Recombinant Chinese Hamster Ovary Cells

      • Mark A. Gerber, Kimberly A. Lacy, Jennifer Cresswell, Nan Lin, Kevin J. Kayser, Matthew V. Caple

      Pages 35-39

   9. An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines

      • Genova A. Richardson, Daniel W. Allison, Nan Lin, Matthew V. Caple, Kevin J. Kayser

      Pages 41-44

   10. Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System

       • Genova A. Richardson, Nan Lin, Kimberly A. Lacy, Lynn A. Davis, Misa I. Gray, Jennifer Cresswell et al.

       Pages 45-48

   11. CHO-DG44 Cell Line Development by FLP-Targeting – High Level Glycoprotein Expression with Significantly Decreased Time Lines

       • Thomas Rose, Anne Furthmann, Marie Etzien, Karsten Winkler, Volker Sandig

       Pages 49-55

   12. Transgene Copy Number – Impact on Clone Performance

       • Karsten Winkler, Thomas Rose, Anne Furthmann, Christine Paul, Andrea Franke, Rita Berthold et al.

       Pages 57-62

   13. Single Use Bioreactors: Expressing Protein in Mammalian Cell Suspension

       • Kevin A. Auton

       Pages 63-69

3. Applied Integrative Physiology

   1. Front Matter

      Pages 71-71

      PDF

   2. Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseases

      • M. Grez, S. Stein, U. Siler, R. Seger

      Pages 73-78

   3. An Avian Cell Line for Production of Highly Attenuated Viral Vectors

      • Ingo Jordan, Deborah Horn, Kristin Höwing, Volker Sandig

      Pages 79-88

   4. Microelectronic Cellular Vitality Monitoring

      • M. Schmidhuber, J. Wiest, A.M. Otto, M. Brischwein, H. Grothe, B. Wolf

      Pages 89-93

   5. IFN-γ Glycosylation Macroheterogeneity, Energetic Cell Status and Medium Composition During CHO Cell Cultures

      • N. Kochanowski, F. Blanchard, R. Cacan, F. Chirat, E. Guedon, A. Marc et al.

      Pages 95-99

   6. Modelling of Neural Metabolism Using 13C-NMR Spectroscopy and Metabolic Flux Analysis

      • Ana P. Teixeira, Sónia Sá Santos, Ana I. Amaral, Nuno Carinhas, Rui Oliveira, Manuel J.T. Carrondo et al.

      Pages 101-106

Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.

• Proteomics
• bioinformatics
• biotechnology
• cell
• cell culture
• glycoprotein
• microarray
• physiology
• protein
• proteins

• Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

  Thomas Noll

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