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Cells and Culture

Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007

  • Conference proceedings
  • © 2010

Overview

Editors:
  1. Thomas Noll

    1. Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

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  • A Comprehensive review of the latest research and developments in the field of animal cell technology.
  • A unique collection of papers on animal cell technology for industrialists.
  • Practical information on the applications of animal cell technology for the production of biomedicines

Part of the book series: ESACT Proceedings (ESACT, volume 4)

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Table of contents (152 papers)

  1. Front Matter

    Pages i-liv
    Download chapter PDF

  2. Single-Cell Analysis and Engineering

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells

      • Trissa Borgschulte, Fanny Delegrange, Christoph L. Bausch, David L. Hacker, Daniel W. Allison, Matthew V. Caple et al.
      Pages 3-8

    3. RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses

      • Peter de Haan, Alfred Engel, Walter de Vries, Jeroen van der Velden, Gerrit-Jan van Holst, Eddy Arnoldussen
      Pages 9-12

    4. Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in a 3-D Scaffold for Tissue Engineering

      • Eva-Maria Engelhardt, A. Oberbek, D. Aibibu, G. Backliwal, M. Adam, J. Hilborn et al.
      Pages 13-18

    5. Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element

      • Sarah Wulhfard, Stéphanie Tissot, David L. Hacker, Florian M. Wurm
      Pages 19-23

    6. Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production

      • Josef Schulze-Horsel, Mareike Schulze, Yvonne Genzel, Udo Reichl
      Pages 25-28

    7. Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation

      • Christian Weber, Stephanie Gokorsch, Peter Czermak
      Pages 29-34

    8. Cell Xpress™-Assisted Analysis of Clone Stability in Recombinant Chinese Hamster Ovary Cells

      • Mark A. Gerber, Kimberly A. Lacy, Jennifer Cresswell, Nan Lin, Kevin J. Kayser, Matthew V. Caple
      Pages 35-39

    9. An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines

      • Genova A. Richardson, Daniel W. Allison, Nan Lin, Matthew V. Caple, Kevin J. Kayser
      Pages 41-44

    10. Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System

      • Genova A. Richardson, Nan Lin, Kimberly A. Lacy, Lynn A. Davis, Misa I. Gray, Jennifer Cresswell et al.
      Pages 45-48

    11. CHO-DG44 Cell Line Development by FLP-Targeting – High Level Glycoprotein Expression with Significantly Decreased Time Lines

      • Thomas Rose, Anne Furthmann, Marie Etzien, Karsten Winkler, Volker Sandig
      Pages 49-55

    12. Transgene Copy Number – Impact on Clone Performance

      • Karsten Winkler, Thomas Rose, Anne Furthmann, Christine Paul, Andrea Franke, Rita Berthold et al.
      Pages 57-62

    13. Single Use Bioreactors: Expressing Protein in Mammalian Cell Suspension

      • Kevin A. Auton
      Pages 63-69

  3. Applied Integrative Physiology

    1. Front Matter

      Pages 71-71
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    2. Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseases

      • M. Grez, S. Stein, U. Siler, R. Seger
      Pages 73-78

    3. An Avian Cell Line for Production of Highly Attenuated Viral Vectors

      • Ingo Jordan, Deborah Horn, Kristin Höwing, Volker Sandig
      Pages 79-88

    4. Microelectronic Cellular Vitality Monitoring

      • M. Schmidhuber, J. Wiest, A.M. Otto, M. Brischwein, H. Grothe, B. Wolf
      Pages 89-93

    5. IFN-γ Glycosylation Macroheterogeneity, Energetic Cell Status and Medium Composition During CHO Cell Cultures

      • N. Kochanowski, F. Blanchard, R. Cacan, F. Chirat, E. Guedon, A. Marc et al.
      Pages 95-99

    6. Modelling of Neural Metabolism Using 13C-NMR Spectroscopy and Metabolic Flux Analysis

      • Ana P. Teixeira, Sónia Sá Santos, Ana I. Amaral, Nuno Carinhas, Rui Oliveira, Manuel J.T. Carrondo et al.
      Pages 101-106
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Keywords

About this book

Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.

Editors and Affiliations

  • Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

    Thomas Noll

Bibliographic Information

  • Book Title: Cells and Culture

  • Book Subtitle: Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007

  • Editors: Thomas Noll

  • Series Title: ESACT Proceedings

  • DOI: https://doi.org/10.1007/978-90-481-3419-9

  • Publisher: Springer Dordrecht

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Science+Business Media B.V. 2010

  • Hardcover ISBN: 978-90-481-3418-2Published: 14 September 2010

  • Softcover ISBN: 978-94-024-0520-0Published: 23 August 2016

  • eBook ISBN: 978-90-481-3419-9Published: 17 July 2010

  • Edition Number: 1

  • Number of Pages: LIV, 800

  • Topics: Biomedicine general

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