Logo - springer
Slogan - springer

Biomedical Sciences | Cell Line Development

Cell Line Development

Series: Cell Engineering, Vol. 6

Al-Rubeai, Mohamed (Ed.)

2009

Available Formats:
eBook
Information

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.

 
$229.00

(net) price for USA

ISBN 978-90-481-2245-5

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase


learn more about Springer eBooks

add to marked items

Hardcover
Information

Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.

 
$289.00

(net) price for USA

ISBN 978-90-481-2244-8

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days


add to marked items

Softcover
Information

Softcover (also known as softback) version.

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.

 
$289.00

(net) price for USA

ISBN 978-94-007-3040-3

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days


add to marked items

  • Presents an overall picture of the current state of knowledge in Cell Line Development
  • Designed to offer a state-of-the-art review with in–depth assessments and perspectives of expression engineering through understanding the molecular and cellular basis of cell culture systems
  • The various chapters describe the necessary methodologies for using cell engineering tools for the improvement of cell culture processes
  • Describes the most recent methods used to establish mammalian host cell lines and expression systems, optimisation of selection systems to reduce development times and improvement of glycosylation and secretion

Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Content Level » Research

Keywords » Activation - CMV - Expression - RNA - biotechnology - gene expression - methodology - protein - proteins - transgen - translation

Related subjects » Biochemistry & Biophysics - Biomedical Sciences - Biotechnology - Cell Biology

Table of contents 

1. Use of MAR elements to increase the production of recombinant proteins, Cori Gorman, Salina Arope, Mélanie Grandjean, Pierre-Alain Girod, and Nicolas Mermod; 2. Expression Engineering – the IE2 promoter/enhancer from mouse CMV, Markus O. Imhof; 3. Defeating Randomness – Targeted Integration as a Boost for Biotechnology, Gama-Norton, L.; Riemer, P.; Sandhu, U.; Nehlsen, K.; Schucht, R.; Hauser, H. and Wirth D.; 4. Importance of genetic environment for recombinant gene expression, Alan J. Dickson; 5. Expression Vector Engineering for Recombinant Protein Production, Helen Kim, John Laudemann, Jennitte Stevens and Michelle Wu; 6. Cell XpressTM Applications in Development and Characterization of Biopharmaceutical Recombinant Protein Producing Cell Lines, Jennifer R. Cresswell, Nan Lin, Genova A. Richardson and Kevin J. Kayser; 7. Selection Methods for High-Producing Mammalian Cell Lines, S. M. Browne and M. Al-Rubeai ; 8. Engineering Mammalian Cells for Recombinant Monoclonal Antibody Production, Sarah L. Davies and David C. James; 9. Engineering Cell Function by RNA Interference, Joseph A. Gredell, Hemant K. Kini, and S. Patrick Walton; 10. Apoptosis and Autophagy Cell Engineering, Chaya Mohan, Yeon-Gu Kim, Gyun Min Lee ; 11. Glycoengineering and Modeling of Protein N-Glycosylation, Sandra V. Bennun, Frederick J. Krambeck and Michael J. Betenbaugh; 12. Engineering the Secretory pathway in mammalian cells, Ren-Wang Peng and Martin Fussenegger

Popular Content within this publication 

 

Articles

Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Biomedicine (general).