Rat Experimental Transplantation Surgery

1. Front Matter

   Pages i-x

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2. Laboratory Rats: Anatomical and Physiological Notes

   • Ivan Misek

   Pages 1-13

3. Basic Equipment for Microsurgical Experiment

   • Peter Balaz

   Pages 15-18

4. Immunosuppresive Drugs Commonly Used in Transplantation Models

   • Peter Girman

   Pages 19-29

5. Basics in Rat MHC Genomics

   • Peter Girman

   Pages 31-33

6. Anesthesia in Experimental Microsurgery

   • Ladislav Hess, Hynek Riha

   Pages 35-47

7. Basic Techniques for Microsurgery Experiment

   • Peter Balaz, Jan Kriz

   Pages 49-66

8. Recovery After Surgery

   • Eva Fabryova, Jan Kriz

   Pages 67-70

9. Kidney Transplantation

   • Ondrej Viklicky, Eva Girmanova, Peter Balaz

   Pages 71-84

10. Heterotopic Abdominal Heart Transplantation in Rats

    • Martin Pokorny, Iveta Mrazova, Jiri Maly

    Pages 85-110

11. Orthotopic Experimental Lung Transplantation

    • Andreas Habertheuer, Dominik Wiedemann, Gunther Laufer, Alfred Kocher, Martin Andreas

    Pages 111-144

12. Experimental Liver Transplantation and Surgery

    • Firas Zahr Eldeen, Hynek Mergental

    Pages 145-162

13. The Transplantation of Insulin Producing Tissue

    • Jan Kriz, Peter Girman, Zuzana Berkova, Tomas Marada

    Pages 163-198

14. Small Intestine Transplantation

    • Michal Kudla, Peter Balaz

    Pages 199-213

15. Transplantation of Lymph Vessels

    • Michal Kudla

    Pages 215-225

16. Transplantation of Vascular Allograft

    • Ivan Matia, Martin Varga, Peter Fellmer

    Pages 227-248

17. Back Matter

    Pages 249-252

    PDF

The aim of the book is to describe tested microsurgical procedures of kidney, pancreas, islets, heat, liver and small bowel transplantation. All procedures written in the book are used in our experimental research laboratory and their description will be provided by an experienced researcher. The book is organized into ‘General’ and ‘Specific’ sections. The ‘General’ section will include principles, doses and available drugs for rat anaesthesia, the surgical anatomy of the rat, a brief review of immunosuppressant’s used in rat models, a description of basic surgical techniques and blood sampling. The ‘Specific’ section will include a description of the rat model with the appropriate organ failure relevant to the organ transplantation, which will be followed by a detailed description of the surgical procedure with high quality pictures of key steps. Each chapter will describe ‘tips and tricks’ including practical advice and recommendations.

• animal models
• microsurgery
• rat
• statistical models
• tissue banking
• transplantation

“This is a fundamental book for researchers interested in starting or continuing work in an animal laboratory. … The book is of high quality and I imagine it would be of great benefit to any students and researchers participating in these procedures. I am sure it would be helpful to people involved in animal research that isn’t necessarily even related to transplant.” (Krishna G, Putchakayala, Doody’s Book Reviews, March, 2016)

• Diabetes Center, Institute for Clinical Experimental Med. , Prague, Czech Republic

  Peter Girman

• Pancreatic Islet Lab. Diabetes Center, Institute for Clinical Experimental Med. , Prague, Czech Republic

  Jan Kriz

• Dept. of Transplantation Surgery, Institute for Clinical Experimental Med. , Prague, Czech Republic

  Peter Balaz

Policies and ethics