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Advanced Methods in Structural Biology

  • Book
  • © 2016

Overview

Editors:
  1. Toshiya Senda

    1. Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) , Tsukuba, Japan

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  2. Katsumi Maenaka

    1. Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • Explains difficulties in structural biology and provides methods to overcome these difficulties with protocols and theories behind them
  • Offers not only experimental methods (protocols and examples of protein experiments) but also the theoretical background of each method.
  • Includes abundant references and practical examples with useful figures

Part of the book series: Springer Protocols Handbooks (SPH)

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About this book

This book provides practical information on a whole set of protein experiments for advanced structural biology, such as X-ray crystallography, NMR, electron microscopy, advanced mass spectroscopy, and surface plasmon resonance, as well as a wide variety of expression systems including eukaryotic and in vitro expression. In the past decade, structural genomics studies have pushed forward the development of automated methods in the field of structural biology, however there is an increasing need for the structural analysis of difficult targets, such as large protein complexes and membrane proteins, which are hard to achieve using conventional automated methods, and require knowledge that goes beyond standard protein chemistry protocols. To address these problems and to help researchers develop novel methods, this volume provides examples of the development of new protein investigation methods and their theoretical background.This book particularly appeals to graduate students, postdoctoral researchers, young investigators wishing to gain a better understanding of the theory behind experiments, and those seeking further advanced, practical structural biology methods.

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
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  2. Protein Expression

    1. Front Matter

      Pages 1-1
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    2. Expression in Bacteria and Refolding

      • Hiroki Akiba, Kouhei Tsumoto
      Pages 3-23

    3. Expression of Proteins in Insect and Mammalian Cells

      • Shunsuke Kita, Katsumi Maenaka, Takashi Tadokoro
      Pages 25-45

    4. Application of MultiBac System to Large Complexes

      • Shuya Fukai
      Pages 47-65

    5. Purification Using Affinity Tag Technology

      • Atsushi Furukawa, Katsumi Maenaka, Takao Nomura
      Pages 67-81

    6. Cell-Free Protein Production for Structural Biology

      • Takaho Terada, Seisuke Kusano, Takayoshi Matsuda, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 83-102

  3. Purification and Crystallization of Membrane Proteins

    1. Front Matter

      Pages 103-103
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    2. Overview of Membrane Protein Purification and Crystallization

      • Tatsuro Shimamura
      Pages 105-122

    3. Cell-Free Synthesis of Membrane Proteins

      • Tomomi Kimura-Someya, Toshiaki Hosaka, Takehiro Shinoda, Kazumi Shimono, Mikako Shirouzu, Shigeyuki Yokoyama
      Pages 123-135

  4. Crystallization and Crystal Engineering

    1. Front Matter

      Pages 137-137
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    2. Screening of Cryoprotectants and the Multistep Soaking Method

      • Miki Senda, Toshiya Senda
      Pages 139-151

    3. Protein Modification for Crystallization

      • Toshio Hakoshima
      Pages 153-161

  5. Interaction Analysis

    1. Front Matter

      Pages 163-163
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    2. Analytical Ultracentrifugation

      • Elena Krayukhina, Susumu Uchiyama
      Pages 165-183

    3. Mass Spectrometry

      • Masanori Noda, Kiichi Fukui, Susumu Uchiyama
      Pages 185-198

    4. Frontal Gel Filtration

      • Tetsuo Ishida
      Pages 199-226

    5. Surface Plasmon Resonance

      • Yoshihiro Kobashigawa, Natsuki Fukuda, Yusuke Nakahara, Hiroshi Morioka
      Pages 227-237

  6. Advanced Methods for Structural Analyses

    1. Front Matter

      Pages 239-239
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    2. Structural Biology with Microfocus Beamlines

      • Kunio Hirata, James Foadi, Gwyndaf Evans, Kazuya Hasegawa, Oliver B. Zeldin
      Pages 241-273
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Editors and Affiliations

  • Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) , Tsukuba, Japan

    Toshiya Senda

  • Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

    Katsumi Maenaka

Bibliographic Information

  • Book Title: Advanced Methods in Structural Biology

  • Editors: Toshiya Senda, Katsumi Maenaka

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-4-431-56030-2

  • Publisher: Springer Tokyo

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Japan 2016

  • Hardcover ISBN: 978-4-431-56028-9Published: 03 August 2016

  • Softcover ISBN: 978-4-431-56758-5Published: 07 June 2018

  • eBook ISBN: 978-4-431-56030-2Published: 20 July 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 340

  • Number of Illustrations: 31 b/w illustrations, 65 illustrations in colour

  • Topics: Protein Science

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