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  • Textbook
  • © 2012

The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications

  • Detailed protocols on Angiogenesis/Lymphangiogenesis techniques Emphasis in color illustrations Up-to-date comprehensive compilation of Angiogenesis/Lymphangiogenesis techniques.
  • Includes supplementary material: sn.pub/extras

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Table of contents (26 chapters)

  1. Front Matter

    Pages i-vii
  2. The In Vitro Endothelial Cell Tube Formation Assay in 3D Culture on Gelled Basement Membrane Extract

    • Irina Arnaoutova, Hynda K. Kleinman, Jay George, Gabriel Benton
    Pages 1-10
  3. Electron Microscopy in Angiogenesis Research

    • Ruth M. Hirschberg, Johanna Plendl
    Pages 33-68
  4. Methods to Assess Vascular Permeability During Angiogenic Processes

    • Alexander N. Garcia, Joe G. N. Garcia
    Pages 69-83
  5. Quantitative Methods to Study Adipose Angiogenesis

    • Sharon Lim, Jennifer Honek, Ziquan Cao, Takahiro Seki, Yuan Xue, Yihai Cao
    Pages 85-99
  6. Methodologic Approaches to Investigate Vascular Tube Morphogenesis and Maturation Events in 3D Extracellular Matrices In Vitro and In Vivo

    • Amber N. Stratman, Dae Joong Kim, Anastasia Sacharidou, Katherine R. Speichinger, George E. Davis
    Pages 101-126
  7. Preparation and Analysis of Aortic Ring Cultures for the Study of Angiogenesis Ex Vivo

    • Roberto F. Nicosia, Giovanni Ligresti, Alfred C. Aplin
    Pages 127-148
  8. The Chick Embryo Aortic Arch Assay

    • Robert Auerbach, Veerappan Muthukkaruppan
    Pages 149-157
  9. The P-Sp Culture System

    • Nobuyuki Takakura
    Pages 159-168
  10. Ex Vivo Retinal Explant Cultures to Study Angiogenic Responses

    • Suphansa Sawamiphak, Ioanna Bethani, Mathias Ritter, Amparo Acker-Palmer
    Pages 169-180
  11. The Mouse Model of Oxygen-Induced Retinopathy (OIR)

    • Andreas Stahl, Jing Chen, Jean-Sebastian Joyal, Lois E. H. Smith
    Pages 181-188
  12. Quantitative Analysis of Angiogenesis in the Allantois Explant Model

    • Laure Gambardella, Enrique Zudaire, Sonja Vermeren
    Pages 189-204
  13. Developing an Ex Vivo Model of Ischemia Using Early Chick-Embryo: A Model to Study Ischemia Related Angiogenesis

    • Syamantak Majumder, Sree Rama Chaitanya Sridhara, Suvro Chatterjee
    Pages 241-251
  14. The Zebrafish/Tumor Xenograft Angiogenesis Assay

    • Marco Presta, Giulia De Sena, Chiara Tobia
    Pages 253-268
  15. Quantitative Study of In Vivo Angiogenesis and Vasculogenesis Using Matrigel-Based Assays

    • Kaustabh Ghosh, Mehrdad Khajavi, Avner Adini
    Pages 269-283
  16. Corneal Pocket Assay

    • Marina Ziche, Lucia Morbidelli
    Pages 285-304
  17. Directed In Vivo Angiogenesis Assay (DIVAA) for the Screening of Angiogenesis Modulators

    • Liliana Guedez, William G. Stetler-Stevenson
    Pages 305-309

About this book

Angiogenesis and lymphangiogenesis have become attractive targets for drug therapy because of their key roles in a broad spectrum of pathological disease states ranging from macular degeneration to tumor growth and metastasis. A substantial increase in the research effort over the past decade has deepened our understanding of the basic mechanisms underlying angiogenesis and lymphangiogenesis, promoting the development of promising therapeutics for the clinical management of vascular-related diseases. These extraordinary advancements have been built upon a vast array of diverse analytical techniques developed globally throughout the field. Over the years, these methods have evolved to suit the specific needs of different researchers and experimental scenarios, resulting in a myriad of technical variants of basic assay approaches.

"The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications" is an up-to-date comprehensive textbook on angiogenesis and lymphangiogenesis techniques and applications. This volume is designed to embody the collective works of experts in the clinical as well as the basic research arenas who have significantly contributed to the development and application of techniques in all areas of angiogenesis and lymphangiogenesis. Each chapter introduces and discusses one or a group of closely related techniques and convey step-by-step protocol information and detailed technical guidance to the reader. Emphasis has been placed on explanatory illustrations, critical technical steps as well as divulging information on the benefits and caveats of specific practices related to the methods discussed. This manual is intended to serve as a written guide for both newcomers and established professionals in the field.

Editors and Affiliations

  • Radiation Oncology, National Cancer Institute, Angiogenesis Core Facility, Gaithersburg, USA

    Enrique Zudaire, Frank Cuttitta

Bibliographic Information

  • Book Title: The Textbook of Angiogenesis and Lymphangiogenesis: Methods and Applications

  • Editors: Enrique Zudaire, Frank Cuttitta

  • DOI: https://doi.org/10.1007/978-94-007-4581-0

  • Publisher: Springer Dordrecht

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Science+Business Media Dordrecht 2012

  • Hardcover ISBN: 978-94-007-4580-3Published: 28 November 2012

  • Softcover ISBN: 978-94-007-9783-3Published: 14 December 2014

  • eBook ISBN: 978-94-007-4581-0Published: 29 November 2012

  • Edition Number: 1

  • Number of Pages: VIII, 440

  • Topics: Biomedicine general, Cancer Research, Angiology, Cell Culture

Buy it now

Buying options

eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access