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Electroporation and Sonoporation in Developmental Biology

  • Book
  • © 2009

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Editors:
  1. Harukazu Nakamura

    1. Graduate School of Life Sciences/Institute of Development, Aging & Cancer, Tohoku University, Aoba-ku, Japan

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Table of contents (27 chapters)

  1. Front Matter

    Pages i-xxix
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  2. Electroporation

    1. Short History of Electroporation for the Study of Developmental Biology

      • Harukazu Nakamura
      Pages 3-7

    2. In Ovo Electroporation as a Useful Tool to Pursue Molecular Mechanisms of Neural Development in Chick Embryos

      • Noritaka Odani, Xubin Hou, Harukazu Nakamura
      Pages 9-16

    3. Applications of Tol2 Transposon-Mediated Gene Transfer for Stable Integration and Conditional Expression of Electroporated Genes in Chicken Embryos

      • Yuki Sato, Yoshiko Takahashi
      Pages 17-24

    4. Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systems in Vertebrate Embryos

      • Julien Dubrulle, Olivier Pourquié
      Pages 25-36

    5. In Ovo Electroporation for Targeting the Somitic Mesoderm

      • Emi Ohata, Yoshiko Takahashi
      Pages 37-42

    6. Method of Electroporation for the Early Chick Embryo

      • Jun Hatakeyama, Kenji Shimamura
      Pages 43-53

    7. Enhancer Analysis

      • Masanori Uchikawa, Tatsuya Takemoto
      Pages 55-71

    8. Electroporation of Nucleic Acids into Chick Endoderm Both In Vitro and In Ovo

      • Kimiko Fukuda
      Pages 73-83

    9. Electroporation into the Limb: Beyond Misexpression

      • Takayuki Suzuki, Toshihiko Ogura
      Pages 85-96

    10. Retinal Fiber Tracing by In Ovo Electroporation

      • Hidekiyo Harada, Harukazu Nakamura
      Pages 97-104

    11. Retroviral Vector-Mediated Gene Transfer into the Chick Optic Vesicle by In Ovo Electroporation

      • Hiraki Sakuta, Ryoko Suzuki, Masaharu Noda
      Pages 105-116

    12. Clonal and Widespread Gene Transfer by Proviral Electroporation for Analysis of Brain Laminar Formation

      • Sayaka Sugiyama, Harukazu Nakamura
      Pages 117-127

    13. Electroporation into Cultured Mammalian Embryos

      • Tadashi Nomura, Masanori Takahashi, Noriko Osumi
      Pages 129-141

    14. In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neurons

      • Hidenori Tabata, Kazunori Nakajima
      Pages 143-152

    15. Practical Application of Microelectroporation into Developing Mouse Brain

      • Tomomi Shimogori, Masaharu Ogawa
      Pages 153-167

    16. Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures

      • Naofumi Uesaka, Yasufumi Hayano, Akito Yamada, Nobuhiko Yamamoto
      Pages 169-177

    17. Temporal and Tissue-Specific Control of Gene Expression in the Peri-Implantation Mouse Embryo Through Electroporation of dsRNA

      • Miguel L. Soares, Maria-Elena Torres-Padilla
      Pages 179-200

    18. Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderm Determination Towards a Haematopoietic Fate

      • Anna-Lila Kaushik, Sébastien J. D. Giroux, Michèle Klaine, Ali Jalil, Yann Lécluse, Isabelle Godin
      Pages 201-217

    19. Electroporation of Embryonic Kidney Explants

      • Nicholas Haddad, Daniel Houle, Indra R. Gupta
      Pages 219-227
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Keywords

About this book

In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.

Editors and Affiliations

  • Graduate School of Life Sciences/Institute of Development, Aging & Cancer, Tohoku University, Aoba-ku, Japan

    Harukazu Nakamura

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