Gene Vaccines

1. Front Matter

   Pages i-xi

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2. General Mechanisms of Gene Vaccines

   • Richard Weiss, Viggo Van Tendeloo, Sandra Scheiblhofer, Josef Thalhamer

   Pages 1-35

3. Strategies to Improve DNA Vaccine Potency: HPV-Associated Cervical Cancer as a Model System

   • Chien-Fu Hung, Barbara Ma, Yijie Xu, T.-C. Wu

   Pages 37-65

4. Immune-Activating Mechanisms of Replicase-Based DNA and RNA Vaccines and Their Role in Immune-Apoptosis

   • Wolfgang W. Leitner

   Pages 67-88

5. Cytokine Genes as Molecular Adjuvants for DNA Vaccines

   • Bin Wang, Youmin Kang, Richard Ascione

   Pages 89-107

6. Pharmaceutical Non-Viral Formulations for Gene Vaccines

   • Glen Perera, Andreas Bernkop-Schnürch

   Pages 109-125

7. Rational Design of Formulated DNA Vaccines: The DermaVir Approach

   • Eszter Nátz, Julianna Lisziewicz

   Pages 127-143

8. Improvement of DNA Vaccines by Electroporation

   • Arielle A. Ginsberg, Xuefei Shen, Natalie A. Hutnick, David B. Weiner

   Pages 145-162

9. Electroporation Based TriGrid™ Delivery System (TDS) for DNA Vaccine Administration

   • Drew Hannaman

   Pages 163-181

10. Prime Boost Regimens for Enhancing Immunity: Magnitude, Quality of Mucosal and Systemic Gene Vaccines

    • Danushka K. Wijesundara, Charani Ranasinghe

    Pages 183-204

11. Intralymphatic Vaccination

    • Thomas M. Kündig, Adrian Bot, Gabriela Senti

    Pages 205-221

12. Messenger RNA Vaccines

    • Jochen Probst, Mariola Fotin-Mleczek, Thomas Schlake, Andreas Thess, Thomas Kramps, Karl-Josef Kallen

    Pages 223-245

13. DNA and RNA Vaccines for Prophylactic and Therapeutic Treatment of Type I Allergy

    • Richard Weiss, Sandra Scheiblhofer, Elisabeth Rösler, Josef Thalhamer

    Pages 247-263

14. Approved Veterinary Vaccines: Paving the Way to Products for Human Health

    • Matthias Giese

    Pages 265-283

15. Current Status of Regulations for DNA Vaccines

    • Bettina Klug, Jens Reinhardt, James Robertson

    Pages 285-295

16. Minicircle-DNA

    • Peter Mayrhofer, Michaela Iro

    Pages 297-310

17. Industrial Manufacturing of Plasmid-DNA Products for Gene Vaccination and Therapy

    • Jochen Urthaler, Hermann Schuchnigg, Patrick Garidel, Hans Huber

    Pages 311-330

The induction of antigen-specific immune responses after in vivo transfection with expression plasmids has triggered a revolution of vaccine research. After a first hype, evoked by the fascinating options of this method, clinical studies did not reach the ambitious aims and a phase of disillusion ensued. It became obvious that Gene vaccines displayed a weaker immunogenicity in humans than had been observed in the mouse models. Meanwhile these hurdles have been overcome and gene vaccines undergo a renaissance. The present book gives an update of the “world of naked gene vaccines”, namely DNA and RNA vaccines. Its content ranges from general mechanisms, inherent immunostimulatory properties and the vast potential to modulate immune responses, to recent successful clinical studies and approved veterinary gene vaccines. Beyond the state-of-the-art of genetic immunization, the reader will be stimulated with a chapter addressing “burning questions”.

• RNA and DNA vaccines
• antigen-specific
• gene vaccines
• immune response
• vaccination
• vaccines

• , Department of Molecular Biology, University of Salzburg, Salzburg, Austria

  Josef Thalhamer, Richard Weiss, Sandra Scheiblhofer

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