Skip to main content
SpringerLink
Log in
Book cover

Nonradioactive Analysis of Biomolecules

  • Book
  • © 2000

Overview

Editors:
  1. Christoph Kessler

    1. Roche Molecular Systems Werk Tutzing, Roche Diagnostics GmbH, Tutzing, Germany
      Gene Center/Institute of Biochemistry, Ludwig-Maximilians-Universität München, München, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • A practical bench-top guide with over 50 up-to-date protocols for a wide range of research areas
  • Clearly structured protocols, including step-by-step procedures, troubleshooting sections and practical hints
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (65 chapters)

  1. Front Matter

    Pages I-XVII
    Download chapter PDF

  2. Introduction

    1. General Aspects of Nonradioactive Analysis of Biomolecules

      • Christoph Kessler
      Pages 1-37

  3. Standard Nonradioactive Labeling Systems

    1. Front Matter

      Pages 39-39
      Download chapter PDF

    2. Overview on Nonradioactive Labeling Systems

      • Christoph Kessler
      Pages 41-58

    3. The DIG (Digoxigenin:Anti-Digoxigenin) System

      1. Overview on the Digoxigenin: Anti-Digoxigenin (DIG) System
        • Christoph Kessler
        Pages 59-60
      2. Labeling and Detection of Nucleic Acids
        • Hans-Joachim Höltke, Kelly King, Cathy Hoover, Irmgard Obermaier, Barbara RÜger, Klaus MÜhlegger et al.
        Pages 61-93
      3. Labeling and Detection of Proteins and Glycoproteins
        • Anton Haselbeck, Wolfgang HÖsel
        Pages 94-110

    4. The Biotin (Biotin:Streptavidin) System

      1. Labeling and Detection of Nucleic Acids
        • Ayoub Rashtchian, Jesse Mackey
        Pages 111-136
      2. Labeling of Proteins During <i>In Vitro</i> Translation
        • Thomas Metzler, Hoeltke Hans-Joachim
        Pages 137-154
      3. Labeling and Detection of Proteins and Glycoproteins
        • Edward A. Bayer, Meir Wilchek
        Pages 155-167

    5. 5-BrdU, AAF, Gold Labeling

      1. <i>In Vivo</i>Labeling of DNA Probes with 5-BrdU
        • Jean-Luc Guesdon
        Pages 168-179
      2. AAF-Labeling of DNA and Oligonucleotides
        • Jean-luc Guesdon, Jacques Auriol, Danièle Chevrier
        Pages 180-192
      3. Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Conjugates
        • F. E. M. Peter van de Plas, Jan L. M. Leunissen
        Pages 193-205

    6. Direct Enzyme Label

      1. Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection
        • Ian Durrant
        Pages 206-215
      2. The SNAP System
        • James E. Marich, Jerry L. Ruth
        Pages 216-224

  4. Specialized Nonradioactive Detection Systems

    1. Front Matter

      Pages 225-225
      Download chapter PDF

    2. Overview on Nonradioactive Detection Systems

      • Christoph Kessler
      Pages 227-239

    3. Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems

      • Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
      Pages 240-248

    4. Colorimetric Systems

      1. Indigo/Tetrazolium Dyes
        • Hans-Joachim Guder, Dieter Heindl, Hans-Peter Josel
        Pages 249-252
      2. Azo Dyes
        • Peter SchÜssler, Christoph G. Grevelding, Werner Kunz
        Pages 253-257
Back to top

Keywords

About this book

New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements.

Editors and Affiliations

  • Roche Molecular Systems Werk Tutzing, Roche Diagnostics GmbH, Tutzing, Germany

    Christoph Kessler

  • Gene Center/Institute of Biochemistry, Ludwig-Maximilians-Universität München, München, Germany

    Christoph Kessler

Bibliographic Information

  • Book Title: Nonradioactive Analysis of Biomolecules

  • Editors: Christoph Kessler

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-57206-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Softcover ISBN: 978-3-540-64601-3Published: 11 March 2000

  • eBook ISBN: 978-3-642-57206-7Published: 06 December 2012

  • Edition Number: 2

  • Number of Pages: XVIII, 750

  • Number of Illustrations: 95 b/w illustrations, 8 illustrations in colour

  • Additional Information: Originally published with the title: Nonradioactive Labeling and Detection of Biomolecules

  • Topics: Cell Biology, Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation