Overview
- Describes a new cell analysis platform which combines microfluidic device with mass spectrometry to determine trace chemicals secreted by the cells
- Develops microfluidic devices integrating the functions of cell culture, drug stimulation, and secretion pretreatment
- Invents an energy-saving cell sorting microluidic device, which successfully achieved a separation efficiency of 97%
- Establishes a cell co-culture microfluidic device to study the signal transmission functions between related organisms
- Nominated as an outstanding contribution by Department of Chemistry, Tsinghua University
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (5 chapters)
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Front Matter
Keywords
About this book
This thesis describes a new approach for cell analysis by the rapid developing microfluidic technology. The nominee has made great contributions to develop a new analysis platform which combined microfluidic devices with mass spectrometry to determine the trace compounds secreted by cells. Based on this analysis platform, she studied the specific cell secreting behaviors under controlled microenvironment, of which the secretion compounds were qualified and semi-quantified by mass spectrometry. A novel cell sorting device integrated homogenous porous PDMS membrane was invented to classify cells from real samples based on the size difference. The nominee further studied the signal transmission between different cells, and the signal chemicals were qualitative and quantitative monitored by the analysis platform. This indicates the potential significant application of the new cell analysis platform in medicine screening and early diagnosis.
Authors and Affiliations
Bibliographic Information
Book Title: Studying Cell Metabolism and Cell Interactions Using Microfluidic Devices Coupled with Mass Spectrometry
Authors: Huibin Wei
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-642-32359-1
Publisher: Springer Berlin, Heidelberg
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer-Verlag Berlin Heidelberg 2013
Hardcover ISBN: 978-3-642-32358-4Published: 18 October 2012
Softcover ISBN: 978-3-642-43086-2Published: 09 November 2014
eBook ISBN: 978-3-642-32359-1Published: 17 October 2012
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XVI, 104
Topics: Mass Spectrometry, Nanotechnology and Microengineering, Cell Biology, Analytical Chemistry