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MicroRNA Expression Detection Methods

  • Book
  • © 2010

Overview

Authors:
  1. Zhiguo Wang

    1. Research Center, Montreal Heart Institute, Montreal, Canada

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    You can also search for this author in PubMed   Google Scholar

  2. Baofeng Yang

    1. Dept. Pharmacology, Harbin Medical University, Harbin, China, People's Republic

    View author publications

    You can also search for this author in PubMed   Google Scholar

  • Gives comprehensive descriptions of miRNA expression detection methods

  • Shows applications of miRNA profiling in miRNA research

  • Highlights the potential of miRNA profiling in clinical diagnosis and prognosis

  • Includes supplementary material: sn.pub/extras

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Table of contents (33 chapters)

  1. Front Matter

    Pages i-xx
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  2. General Remarks

    1. Front Matter

      Pages 1-1
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    2. Detection, Profiling, and Quantification of miRNA Expression

      • Zhiguo Wang, Baofeng Yang
      Pages 3-64

  3. miRNA Microarray Methods

    1. Front Matter

      Pages 66-66
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    2. Microarray and Its Variants for miRNA Profiling

      • Zhiguo Wang, Baofeng Yang
      Pages 67-79

  4. Northern Blotting Methods

    1. Front Matter

      Pages 82-82
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    2. Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs

      • Zhiguo Wang, Baofeng Yang
      Pages 83-100

  5. In Situ Hybridization Methods

    1. Front Matter

      Pages 102-102
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    2. In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs

      • Zhiguo Wang, Baofeng Yang
      Pages 103-128

  6. Real-Time RT-PCR Methods

    1. Front Matter

      Pages 130-130
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    2. End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification

      • Zhiguo Wang, Baofeng Yang
      Pages 131-140

    3. miR-Q RT-PCR for miRNA Quantification

      • Zhiguo Wang, Baofeng Yang
      Pages 141-146

    4. Poly(A)-Tailed Universal Reverse Transcription

      • Zhiguo Wang, Baofeng Yang
      Pages 147-151

    5. Multiplexing RT-PCR for High-Throughput miRNA Profiling

      • Zhiguo Wang, Baofeng Yang
      Pages 153-157

    6. miRNA Amplification Profiling (mRAP)

      • Zhiguo Wang, Baofeng Yang
      Pages 159-170

  7. Cloning Methods

    1. Front Matter

      Pages 172-172
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    2. miRNA Serial Analysis of Gene Expression (miRAGE or SAGE)

      • Zhiguo Wang, Baofeng Yang
      Pages 173-188

  8. Nanoparticle Methods

    1. Front Matter

      Pages 190-190
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    2. Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis

      • Zhiguo Wang, Baofeng Yang
      Pages 191-198

    3. Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling

      • Zhiguo Wang, Baofeng Yang
      Pages 199-206
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Keywords

About this book

MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.

Reviews

From the reviews:

“Designed primarily as a reference for life-science researchers, this book enables investigators to make unbiased comparisons in the search for the best technique for their unique application. This book is organized into 11 major parts … . MicroRNA Expression Detection Methods is a concise, logical presentation of the most relevant microRNA detection methods currently in use. As a procedure reference, this work contributes significantly to the field of microRNA research and will serve as a valuable laboratory resource for life-science professionals.” (David Broyles and Sapna K. Deo, Analytical and Bioanalytical Chemistry, Vol. 399, 2011)

Authors and Affiliations

  • Research Center, Montreal Heart Institute, Montreal, Canada

    Zhiguo Wang

  • Dept. Pharmacology, Harbin Medical University, Harbin, China, People's Republic

    Baofeng Yang

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