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Rapid Cycle Real-Time PCR

Methods and Applications

  • Book
  • © 2001

Overview

Editors:
  1. Stefan Meuer

    1. Institut für Immunologie, Ruprecht Karls-Universität Heidelberg, Heidelberg, Germany

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  2. Carl Wittwer

    1. Department of Pathology, University of Utah Medical School, Salt Lake City, USA

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  3. Kan-Ichi Nakagawara

    1. Sendai, Japan, Miyagino-ku, Sendai, Japan

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Table of contents (43 chapters)

  1. Front Matter

    Pages I-XI
    Download chapter PDF

  2. Rapid Cycle Real-Time PCR: Methods and Applications

    1. Rapid Cycle Real-Time PCR: Methods and Applications

      • Carl Wittwer
      Pages 1-8

  3. Methods

    1. Front Matter

      Pages 9-9
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    2. Mutation Detection by Fluorescent Hybridization Probe Melting Curves

      • Philip S. Bernard, Astrid Reiser, Gregory H. Pritham
      Pages 11-19

    3. Quantification on the LightCycler

      • Randy Rasmussen
      Pages 21-34

    4. Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis

      • Olfert Landt
      Pages 35-41

    5. Using the Nearest Neighbor Model for the Estimation of Matched and Mismatched Hybridization Probe Melting Points and Selection of Optimal Probes on the LightCycler

      • Nicolas von Ahsen, Ekkehard Schütz
      Pages 43-56

    6. Quantification of Human Papilloma Virus Type 16 Using Quantitative Competitive PCR on the LightCycler

      • Brian Erich Caplin
      Pages 57-64

    7. Use of TaqStart Antibody to Increase the Sensitivity of Herpesvirus Quantitative PCR on the LightCycler

      • Karen Brengel-Pesce, Gérard Bargues, Patrice Morand, Jean-Marie Seigneurin
      Pages 65-71

  4. Genotyping of Human Germline Variations

    1. Front Matter

      Pages 73-74
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    2. High-Speed Detection of α1-Antitrypsin Deficiency Alleles Pi*S and Pi*Z on the LightCycler

      • Charalampos Aslanidis, Gerd Schmitz
      Pages 75-81

    3. High-Speed Methylenetetrahydrofolate Reductase C → T 677 Mutation Detection on the LightCycler

      • Charalampos Aslanidis, Gerd Schmitz
      Pages 83-89

    4. Dual Color Detection of Splice Variants of the c-erbA α (Thyroid Hormone Receptor α) Gene

      • Onno Bakker
      Pages 91-96

    5. Detection of Three Major Polymorphisms in the N-Acetyltransferase 2 Gene by Melting Peak Analysis Using Fluorogenic Hybridization Probes

      • Brunhilde Blömeke
      Pages 97-103

    6. Genotyping of Cytochrome P450 2D6*4 Mutation with Fluorescent Hybridization Probes Using LightCycler

      • Jeanette Bjerke, Chung-Che Chang, Chuck Schur, Steven Wong, Naziha Nuwayhid
      Pages 105-110

    7. Fluorescent Hybridization Probe Detection of the F508del Cystic Fibrosis Allele on the LightCycler

      • Cameron N. Gundry
      Pages 111-117

    8. Genotyping β-globin Mutations (Hb S, Hb C, Hb E) by Multiplexing Probe Color and Melting Temperature

      • Mark G. Herrmann
      Pages 119-125

    9. Simultaneous Detection of C282Y and H63D Hemochromatosis Mutations Using LCRed 640 and LCRed 705 Labeled Hybridization Probes

      • Cindy A. Meadows, Marec Phillips, Ming Y. Huang, Elaine Lyon
      Pages 127-134

    10. Genotyping of Angiotensin-Converting Enzyme and Angiotensinogen Polymorphisms with the LightCycler System

      • Eiichi Sakai, Minori Tajima, Mitsuko Mori, Reiko Inage, Manabu Fukumoto, Kan-Ichi Nakagawara
      Pages 135-142

    11. Genotyping of the Most Common Thiopurine Methyltransferase Mutations with the LightCycler

      • Ekkehard Schütz, Nicolas von Ahsen
      Pages 143-152
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Keywords

About this book

The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.

Editors and Affiliations

  • Institut für Immunologie, Ruprecht Karls-Universität Heidelberg, Heidelberg, Germany

    Stefan Meuer

  • Department of Pathology, University of Utah Medical School, Salt Lake City, USA

    Carl Wittwer

  • Sendai, Japan, Miyagino-ku, Sendai, Japan

    Kan-Ichi Nakagawara

Bibliographic Information

  • Book Title: Rapid Cycle Real-Time PCR

  • Book Subtitle: Methods and Applications

  • Editors: Stefan Meuer, Carl Wittwer, Kan-Ichi Nakagawara

  • DOI: https://doi.org/10.1007/978-3-642-59524-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2001

  • eBook ISBN: 978-3-642-59524-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XI, 408

  • Number of Illustrations: 72 b/w illustrations, 70 illustrations in colour

  • Topics: Molecular Medicine

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