Overview
- Detailed step-by-step protocols, allowing immediate application in the lab
- Appealing to geneticists in academic and industrial research but also of interest for labs in the biotech industry
- Shirley Pease heads the "Transgenic and Knockout Core Facility" at the California Institute of Technology, Pasadena, CA; Carlos Lois is at the MIT, Cambridge, MA
- With superb color figures demonstrating the various steps in transgenesis
- Includes supplementary material: sn.pub/extras
Part of the book series: Principles and Practice (PRINCIPLES)
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Table of contents (11 chapters)
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Front Matter
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Back Matter
Keywords
About this book
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.
The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Editors and Affiliations
Bibliographic Information
Book Title: Mammalian and Avian Transgenesis - New Approaches
Editors: Shirley Pease, Carlos Lois
Series Title: Principles and Practice
DOI: https://doi.org/10.1007/978-3-540-28489-5
Publisher: Springer Berlin, Heidelberg
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer-Verlag Berlin Heidelberg 2006
Hardcover ISBN: 978-3-540-28415-4Published: 05 December 2005
Softcover ISBN: 978-3-642-06665-8Published: 12 February 2010
eBook ISBN: 978-3-540-28489-5Published: 31 July 2007
Series ISSN: 1866-914X
Series E-ISSN: 1866-9158
Edition Number: 1
Number of Pages: XX, 281
Number of Illustrations: 36 b/w illustrations, 12 illustrations in colour
Topics: Animal Genetics and Genomics, Cell Biology, Developmental Biology, Molecular Medicine