Overview
- Contains protocols useful for both basic researchers and for those interested in applied viral gene therapy
- Includes viral methods for terminally differentiated cells and for undifferentiated cells, including human stem cells
- Provides example applications for many different viral vectors including HIV, herpes virus, pox virus, cytomegalovirus, adenovirus and adeno-associated viruses, baculovirus, SARS, and many others
- Examines state-of-the-art imaging techniques including in vivo two-color fluorescence video micrsocopy and the combination of fluorescence imaging with PET, SPECT or MRI
- Describes viral applications applicable for Medicine, Neuroscience, Immunology, Agriculture and Laboratory Safety
Part of the book series: Methods in Molecular Biology (MIMB, volume 515)
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Table of contents (24 protocols)
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Front Matter
Keywords
About this book
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
Reviews
From the reviews:
"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) … thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)
"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. … The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. … provides a number of updated protocols for the practicing investigator. … a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody’s Review Service, September, 2009)
“This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants … . The significance of viral applications of fluorescent proteins is well covered … . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences.” (Johannes Schmid, ChemBioChem, Vol. 10, 2009)
“Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. … I enjoyed the book for the breadth of topics and snapshots of research … . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience.” (Paul Monaghan, Infocus Magazine, Issue 19,September, 2010)
Editors and Affiliations
Bibliographic Information
Book Title: Viral Applications of Green Fluorescent Protein
Book Subtitle: Methods and Protocols
Editors: Barry W. Hicks
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-559-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-87-9Published: 01 April 2009
Softcover ISBN: 978-1-4939-6143-6Published: 04 May 2017
eBook ISBN: 978-1-59745-559-6Published: 20 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 357
Number of Illustrations: 46 b/w illustrations, 35 illustrations in colour
Topics: Protein Science, Laboratory Medicine