Overview
- Covers the use of DNA array from the largest commercial providers
- Includes practical details to design and produce your own probes and arrays
- Provides methods for gene expression profiling, SNP mutation analysis, comparative genomic hybridization, resequencing, and ChIP on chip assays
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 529)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (18 protocols)
-
Front Matter
-
Back Matter
Keywords
About this book
DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology.
Bibliographic Information
Book Title: DNA Microarrays for Biomedical Research
Book Subtitle: Methods and Protocols
Editors: Martin Dufva
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-538-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-69-5Published: 27 February 2009
Softcover ISBN: 978-1-61737-941-3Published: 26 January 2011
eBook ISBN: 978-1-59745-538-1Published: 21 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 252
Number of Illustrations: 53 b/w illustrations, 1 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Microarrays