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Auditory and Vestibular Research

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Bernd Sokolowski

    1. University of South Florida, College of Medicine, Tampa, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Lists all the reagents, step-by-step techniques, and limitations for reproducible results in DNA/RNA tracing, protein identification, and microscopic imaging
  • Clearly describes the protocols necessary for in vivo and in vitro gene delivery techniques
  • Provides the tools to identify and isolate cochlear stem cells for the treatment of inner ear pathologies
  • Includes lineage and fate mapping techniques for peripheral and central studies of auditory/vestibular development
  • Describes techniques for identification and verification of protein-protein interactions
  • Provides important tools for the basic and clinical researcher to identify genes and microbes underlying diseases of the ear including the cloning of deafness genes
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 493)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Nucleic Acid Protocols

    1. Front Matter

      Pages 1-1
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    2. RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning

      • Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, Elba E. Serrano
      Pages 3-20

    3. Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays

      • Ana E. Vázquez, Liping Nie, Ebenezer N. Yamoah
      Pages 21-29

    4. In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections

      • Hakim Hiel
      Pages 31-46

    5. Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification

      • Takunori Satoh, Donna M. Fekete
      Pages 47-64

    6. Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus

      • Jun Chul Kim, Susan M. Dymecki
      Pages 65-85

    7. The Practical Use of Cre and loxP Technologies in Mouse Auditory Research

      • Yiling Yu, Jian Zuo
      Pages 87-102

    8. Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium

      • Inna A. Belyantseva
      Pages 103-124

    9. Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear

      • John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth, Catherine S. Bresee
      Pages 125-139

    10. Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear

      • Kazuo Oshima, Pascal Senn, Stefan Heller
      Pages 141-162

    11. Molecular Biology of Vestibular Schwannomas

      • Long-Sheng Chang, D. Bradley Welling
      Pages 163-177

    12. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens

      • Jonathan C. Thomas, Melinda M. Pettigrew
      Pages 179-190

    13. Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa

      • Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner, Garth D. Ehrlich
      Pages 191-213

    14. Positional Cloning of Deafness Genes

      • Hannie Kremer, Frans P.M. Cremers
      Pages 215-239

  3. Amino Acid Protocols

    1. Front Matter

      Pages 239-239
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    2. Twist-Off Purification of Hair Bundles

      • Jung-Bum Shin, James Pagana, Peter G. Gillespie
      Pages 241-255

    3. Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System

      • Dhasakumar S. Navaratnam
      Pages 257-268

    4. The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea

      • Thandavarayan Kathiresan, Margaret C. Harvey, Bernd H. A. Sokolowski
      Pages 269-286

    5. Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis

      • Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge, William E. Brownell
      Pages 287-297
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Keywords

About this book

Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality of sign language, cognitive and language development can be severely impaired in the earliest formative years of a child. In its endeavor to d- cover the mechanisms underlying audition, the ?eld of auditory science has provided rich comparative physiological studies, allowing insights into both the micromecha- cal and electrochemical world of this system. For many years, the auditory/vestibular sciences have been in?uenced by the discoveries of electrical engineers and sensory physiologists, who have provided insights into the functions of this dynamic system. The early discoveries in these ?elds, as well as advancements in microprocessing and materials technologies, provided a means whereby hearing could be regained partly through the use of a bionic device, known as a cochlear implant. Presently, this device and the auditory brainstem implant are the only ones to prosthetically replace brain function. With the advent of molecular biology tools, such as RT-PCR, the auditory and vestibular ?elds have made great strides in understanding the genetic basis for various hearing and balance disorders over the past ?fteen to twenty years. These technologies permitted the discovery of genes that control inner ear structure and function by ov- coming the hurdle of working with small amounts of tissue, as found in the inner ear.

Editors and Affiliations

  • University of South Florida, College of Medicine, Tampa, USA

    Bernd Sokolowski

Bibliographic Information

  • Book Title: Auditory and Vestibular Research

  • Book Subtitle: Methods and Protocols

  • Editors: Bernd Sokolowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-523-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-62-6Published: 23 October 2008

  • Softcover ISBN: 978-1-61737-939-0Published: 20 January 2011

  • eBook ISBN: 978-1-59745-523-7Published: 08 October 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 428

  • Number of Illustrations: 48 b/w illustrations, 13 illustrations in colour

  • Topics: Human Physiology, Neurology, Molecular Medicine, Pathology, Gene Expression

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