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  • © 2009

Type 2 Diabetes

Methods and Protocols

Editors:

  • Provides an intuitive reference volume, written by leading international research groups, for key in vitro and in vivo models used in the study of Type II diabetes
  • Presents a modular approach to experimental design, covering model biological systems, detection technologies, and approaches to data analysis
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 560)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
  2. Nutritional Models of Type 2 Diabetes Mellitus

    • Beverly Sara Mühlhausler
    Pages 19-36
  3. The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans

    • Anna L. Nolan, Jacqueline F. O’Dowd
    Pages 43-51
  4. Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells

    • Dany Muller, Peter M. Jones, Shanta J. Persaud
    Pages 73-86
  5. Laser Capture Microdissection of Human Pancreatic β-Cells and RNA Preparation for Gene Expression Profiling

    • Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, Gordon C. Weir
    Pages 87-98
  6. The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes

    • Nicky Konstantopoulos, Juan Carlos Molero-Navajas
    Pages 111-135
  7. Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas

    • Xiuli Wang, Shundi Ge, Gay M. Crooks
    Pages 191-201
  8. Gene Expression Analysis in Diabetes Research

    • Peter White, Klaus H. Kaestner
    Pages 239-261
  9. Back Matter

    Pages 273-278

About this book

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

Reviews

From the reviews:

“This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists.” (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)

“Written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. … a credible source of information for those seeking experimental advice. … an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher’s bookshelf.” (Robert Michael Sargis, Doody’s Review Service, July, 2010)

Bibliographic Information

  • Book Title: Type 2 Diabetes

  • Book Subtitle: Methods and Protocols

  • Editors: Claire Stocker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-448-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-15-2Published: 10 July 2009

  • Softcover ISBN: 978-1-61779-492-6Published: 30 November 2011

  • eBook ISBN: 978-1-59745-448-3Published: 09 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 49 b/w illustrations, 7 illustrations in colour

  • Topics: Diabetes, Molecular Medicine, Endocrinology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access