Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1114)
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Table of contents (29 protocols)
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Front Matter
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Approaches for Gene Correction from Bacteria to Human Cells
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Front Matter
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Gene Correction Tools Using Non-Viral or Viral Systems
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Front Matter
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Breaking the DNA to Stimulate Gene Correction: Nuclease Design, In Vivo Test, Efficacy, and Off-Target Effect
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Front Matter
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Keywords
About this book
Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.
Editors and Affiliations
Bibliographic Information
Book Title: Gene Correction
Book Subtitle: Methods and Protocols
Editors: Francesca Storici
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-761-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-760-0Published: 21 February 2014
Softcover ISBN: 978-1-4939-6321-8Published: 03 September 2016
eBook ISBN: 978-1-62703-761-7Published: 21 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 463
Number of Illustrations: 28 b/w illustrations, 59 illustrations in colour
Topics: Human Genetics, Gene Function