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  • © 2014

RNA Folding

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of RNA folding, which features in vivo techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and vital implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1086)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. Predicting RNA Structure: Advances and Limitations

    • Ivo L. Hofacker, Ronny Lorenz
    Pages 1-19
  3. RNA Structural Analysis by Enzymatic Digestion

    • Elisa Biondi, Donald H. Burke
    Pages 41-52
  4. The Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA

    • Pablo Cordero, Wipapat Kladwang, Christopher C. VanLang, Rhiju Das
    Pages 53-77
  5. Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes

    • Nora Sachsenmaier, Stefan Handl, Franka Debeljak, Christina Waldsich
    Pages 79-94
  6. Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol

    • Matthew G. Seetin, Wipapat Kladwang, John P. Bida, Rhiju Das
    Pages 95-117
  7. Probing RNA Folding by Hydroxyl Radical Footprinting

    • Maria Costa, Dario Monachello
    Pages 119-142
  8. Chemical Probing of RNA in Living Cells

    • Michael Wildauer, Georgeta Zemora, Andreas Liebeg, Verena Heisig, Christina Waldsich
    Pages 159-176
  9. RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis

    • Sébastien H. Eschbach, Daniel A. Lafontaine
    Pages 255-264
  10. RNA Refolding Studied by Light-Coupled NMR Spectroscopy

    • Harald Schwalbe, Boris Fürtig
    Pages 309-319

About this book

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Editors and Affiliations

  • Dept. of Biochemistry & Cell Biology, University of Vienna Max F. Perutz Laboratories, Vienna, Austria

    Christina Waldsich

Bibliographic Information

  • Book Title: RNA Folding

  • Book Subtitle: Methods and Protocols

  • Editors: Christina Waldsich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-667-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-666-5Published: 18 October 2013

  • Softcover ISBN: 978-1-4939-6058-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-667-2Published: 18 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 340

  • Number of Illustrations: 18 b/w illustrations, 43 illustrations in colour

  • Topics: Human Genetics, Nucleic Acid Chemistry

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access