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  • © 2013

Adhesion Protein Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1046)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Reconstructing Integrin Activation In Vitro

    • Alexandre R. Gingras, Feng Ye, Mark H. Ginsberg
    Pages 1-17
  3. A Multimode-TIRFM and Microfluidic Technique to Examine Platelet Adhesion Dynamics

    • Warwick S. Nesbitt, Francisco J. Tovar-Lopez, Erik Westein, Ian S. Harper, Shaun P. Jackson
    Pages 39-58
  4. Localization-Based Super-Resolution Imaging of Cellular Structures

    • Pakorn Kanchanawong, Clare M. Waterman
    Pages 59-84
  5. Use of Microarray Analysis to Investigate EMT Gene Signatures

    • Andrew H. Sims, Alexey A. Larionov, David J. Harrison, Elad Katz
    Pages 85-95
  6. Podosome Reformation in Macrophages: Assays and Analysis

    • Pasquale Cervero, Linda Panzer, Stefan Linder
    Pages 97-121
  7. Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells

    • Mirjam Andreasson-Ochsner, Erik Reimhult
    Pages 123-132
  8. Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay

    • Marie Schoumacher, Alexandros Glentis, Vasily V. Gurchenkov, Danijela M. Vignjevic
    Pages 133-144
  9. Cytoplasmic Actin: Purification and Single Molecule Assembly Assays

    • Scott D. Hansen, J. Bradley Zuchero, R. Dyche Mullins
    Pages 145-170
  10. Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity

    • Alissa M. Weaver, Jonathan M. Page, Scott A. Guelcher, Aron Parekh
    Pages 171-189
  11. Determining Rho GTPase Activity by an Affinity-Precipitation Assay

    • Narendra Suryavanshi, Anne J. Ridley
    Pages 191-202
  12. Proteomic and Biochemical Methods to Study the Cytoskeletome

    • Richard L. Klemke, Xinning Jiang, Sunkyu Choi, Jonathan A. Kelber
    Pages 203-218
  13. Purification of Native Arp2/3 Complex from Bovine Thymus

    • Lynda K. Doolittle, Michael K. Rosen, Shae B. Padrick
    Pages 231-250
  14. Purification of Arp2/3 Complex from Saccharomyces cerevisiae

    • Lynda K. Doolittle, Michael K. Rosen, Shae B. Padrick
    Pages 251-271
  15. Measurement and Analysis of In Vitro Actin Polymerization

    • Lynda K. Doolittle, Michael K. Rosen, Shae B. Padrick
    Pages 273-293
  16. Use of the xCELLigence System for Real-Time Analysis of Changes in Cellular Motility and Adhesion in Physiological Conditions

    • Simon Scrace, Eric O’Neill, Ester M. Hammond, Isabel M. Pires
    Pages 295-306
  17. Measuring Chemotaxis Using Direct Visualization Microscope Chambers

    • Andrew J. Muinonen-Martin, David A. Knecht, Douwe M. Veltman, Peter A. Thomason, Gabriela Kalna, Robert H. Insall
    Pages 307-321
  18. In Vitro Microtubule Severing Assays

    • Natasza E. Ziółkowska, Antonina Roll-Mecak
    Pages 323-334

About this book

Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.

Editors and Affiliations

  • , Department of Oncology, University of Oxford, Oxford, United Kingdom

    Amanda S. Coutts

Bibliographic Information

  • Book Title: Adhesion Protein Protocols

  • Editors: Amanda S. Coutts

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-538-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-537-8Published: 19 July 2013

  • Softcover ISBN: 978-1-4939-6311-9Published: 23 August 2016

  • eBook ISBN: 978-1-62703-538-5Published: 19 July 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 407

  • Number of Illustrations: 30 b/w illustrations, 57 illustrations in colour

  • Topics: Protein Science, Cell Physiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access