Overview
- Includes cutting-edge methods and protocols exploring the imaging of cellular aspects, tissues, and complete living organisms
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1042)
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Table of contents (23 protocols)
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Front Matter
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Imaging Gene Expression and RNA Dynamics in Cells and Organisms
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Front Matter
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Imaging the Genome and Chromatin Dynamics
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Front Matter
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Imaging Nuclear Processes and Sub-nuclear Structures
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Front Matter
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Keywords
About this book
As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression. In Imaging Gene Expression: Methods and Protocols, expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms.Editors and Affiliations
Bibliographic Information
Book Title: Imaging Gene Expression
Book Subtitle: Methods and Protocols
Editors: Yaron Shav-Tal
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-526-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-525-5Published: 27 August 2013
Softcover ISBN: 978-1-4939-6312-6Published: 30 April 2017
eBook ISBN: 978-1-62703-526-2Published: 27 August 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 368
Number of Illustrations: 18 b/w illustrations, 36 illustrations in colour
Topics: Imaging / Radiology, Gene Expression