Skip to main content
SpringerLink
Log in
Book cover

Trinucleotide Repeat Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Yoshinori Kohwi

    1. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Cynthia T. McMurray

    1. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1010)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction for Trinucleotide Repeat Diseases

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Brief History of Triplet Repeat Diseases

      • Helen Budworth, Cynthia T. McMurray
      Pages 3-17

    3. Huntington’s Disease and Cell Therapies: Past, Present, and Future

      • Chrystalina A. Antoniades, Colin Watts
      Pages 19-32

  3. Analysis of Synaptic Plasticity

    1. Front Matter

      Pages 33-33
      Download chapter PDF

    2. Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice

      • Guang Yang, Feng Pan, Paul C. Chang, Frank Gooden, Wen-Biao Gan
      Pages 35-43

    3. In Vivo Imaging of Synapse Plasticity in the Mouse Motor Cortex

      • Yi Zuo, Xinzhu Yu, Kelly Tennant, Theresa Jones
      Pages 45-57

  4. Targeting on ES Cells

    1. Front Matter

      Pages 59-59
      Download chapter PDF

    2. Pluripotent Hybrid Stem Cells from Transgenic Huntington’s Disease Monkey

      • Chuti Laowtammathron, Anthony W. S. Chan
      Pages 61-77

    3. Mesenchymal Stem Cells for Trinucleotide Repeat Disorders

      • Geralyn Annett, Gerhard Bauer, Jan A. Nolta
      Pages 79-91

  6. Modification of Proteins and DNAs

    1. Front Matter

      Pages 139-139
      Download chapter PDF

    2. Epigenetic Modifications of the FMR1 Gene

      • Elisabetta Tabolacci, Giovanni Neri
      Pages 141-153

    3. The CGG Repeat and the FMR1 Gene

      • Renate K. Hukema, Ben A. Oostra
      Pages 155-176

    4. Study of Oxidative Damage and Antioxidant Systems in Two Huntington’s Disease Rodent Models

      • Francisca Pérez-Severiano, Sergio Montes, Cristian Gerónimo-Olvera, José Segovia
      Pages 177-200

    5. Regulation of Ataxin-1 Phosphorylation and Its Impact on Biology

      • Sarita Lagalwar, Harry T. Orr
      Pages 201-209

  7. Trinucleotide Repeat Expansion and Detection

    1. Front Matter

      Pages 211-211
      Download chapter PDF
Back to top

Keywords

About this book

Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome.  This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.

Editors and Affiliations

  • Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA

    Yoshinori Kohwi, Cynthia T. McMurray

Bibliographic Information

  • Book Title: Trinucleotide Repeat Protocols

  • Editors: Yoshinori Kohwi, Cynthia T. McMurray

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-411-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-410-4Published: 11 June 2013

  • Softcover ISBN: 978-1-4939-5971-6Published: 23 August 2016

  • eBook ISBN: 978-1-62703-411-1Published: 12 June 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 296

  • Number of Illustrations: 40 b/w illustrations, 24 illustrations in colour

  • Topics: Molecular Medicine, Gene Expression

Publish with us

Policies and ethics

Back to top

Search

Navigation