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Organ Regeneration

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Joydeep Basu

    1. Tengion, Inc, Winston-Salem, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. John W. Ludlow

    1. Zen Bio, Inc., Research Triangle Park, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1001)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering

    • Benjamin M. Buehrer, Bentley Cheatham
    Pages 1-11

  3. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs

    • Darell W. McCoy
    Pages 13-20

  4. Isolation of Urothelial Cells from Bladder Tissue

    • Namrata Sangha
    Pages 21-33

  5. Isolation of Pulsatile Cell Bodies from Esophageal Tissue

    • John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, Namrata Sangha
    Pages 35-42

  6. Cell Isolation Through Whole-Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis

    • Sonya O. Meheux
    Pages 43-51

  7. Ex Vivo Culture and Separation of Functional Renal Cells

    • Andrew T. Bruce, Kelly I. Guthrie, Rusty Kelley
    Pages 53-64

  8. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells for Urologic Tissue Engineering

    • Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, Yuanyuan Zhang
    Pages 65-80

  9. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells

    • Tori Sampsell-Barron
    Pages 81-97

  10. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes

    • Gary R. Skuse, Kathleen A. Lamkin-Kennard
    Pages 99-114

  11. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine

    • Teresa Bora Burnette, Andrew T. Bruce
    Pages 115-132

  12. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications

    • Monica A. Serban, Toyin A. Knight, Richard G. Payne
    Pages 133-143

  13. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

    • Adelola O. Oseni, Peter E. Butler, Alexander M. Seifalian
    Pages 145-152

  14. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features

    • Scott Rapoport
    Pages 153-165

  15. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering

    • Richard G. Payne, Toyin A. Knight
    Pages 167-177

  16. Characterization of a PGA-Based Scaffold for Use in a Tissue-Engineered Neo-Urinary Conduit

    • Toyin A. Knight, Richard G. Payne
    Pages 179-188

  17. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications

    • Kelly I. Guthrie, Namrata Sangha, Christopher W. Genheimer, Joydeep Basu, John W. Ludlow
    Pages 189-196

  18. Care of Rodent Models Used for Preclinical Evaluation of Tissue-Engineered/Regenerative Medicine Product Candidates

    • Kim L. Mihalko
    Pages 197-206

  19. Bioreactor Design Considerations for Hollow Organs

    • Jeff Fish, Craig Halberstadt, Darell W. McCoy, Neil Robbins
    Pages 207-214

  20. Construction of a Multicoaxial Hollow Fiber Bioreactor

    • Randall McClelland, Katherine Tech, Jeffrey M. Macdonald
    Pages 215-226
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Keywords

About this book

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.

Editors and Affiliations

  • Tengion, Inc, Winston-Salem, USA

    Joydeep Basu

  • Zen Bio, Inc., Research Triangle Park, USA

    John W. Ludlow

Bibliographic Information

  • Book Title: Organ Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Joydeep Basu, John W. Ludlow

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-363-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-362-6Published: 14 March 2013

  • Softcover ISBN: 978-1-4939-6008-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-363-3Published: 15 March 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 377

  • Topics: Molecular Medicine, Regenerative Medicine/Tissue Engineering

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