Editors:
- Features methodologies and approaches that play an important role in the design cycle for strain improvement
- Provides step-by-step detail essential for reproducible results
- Contains key notes and expert implementation advice in order to guarantee success in the lab
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB)
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Table of contents (23 protocols)
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Front Matter
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Modeling and Simulation Tools
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Front Matter
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Systems-Level ‘Omics Tools
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Front Matter
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Systems-Level ‘Omics Tools
About this book
With the ultimate goal of systematically and robustly defining the specific perturbations necessary to alter a cellular phenotype, systems metabolic engineering has the potential to lead to a complete cell model capable of simulating cell and metabolic function as well as predicting phenotypic response to changes in media, gene knockouts/overexpressions, or the incorporation of heterologous pathways. In Systems Metabolic Engineering: Methods and Protocols, experts in the field describe the methodologies and approaches in the area of systems metabolic engineering and provide a step-by-step guide for their implementation. Four major tenants of this approach are addressed, including modeling and simulation, multiplexed genome engineering, ‘omics technologies, and large data-set incorporation and synthesis, all elucidated through the use of model host organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Systems Metabolic Engineering: Methods and Protocols serves as an ideal guide for metabolic engineers, molecular biologists, and microbiologists aiming to implement the most recent approaches available in the field.
Editors and Affiliations
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Cockrell School of Engineering, Dept. of Chemical Engineering, The University of Texas at Austin, Austin, USA
Hal S. Alper
Bibliographic Information
Book Title: Systems Metabolic Engineering
Book Subtitle: Methods and Protocols
Editors: Hal S. Alper
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-299-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-298-8Published: 16 February 2013
Softcover ISBN: 978-1-4939-6292-1Published: 30 April 2017
eBook ISBN: 978-1-62703-299-5Published: 15 February 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 474
Number of Illustrations: 14 b/w illustrations, 47 illustrations in colour
Topics: Biotechnology, Microengineering, Metabolomics