Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 969)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (19 protocols)
-
Front Matter
-
mRNA Synthesis, Purification, and Transfer
-
Front Matter
-
-
Modulation and Monitoring of Cell Metabolism
-
Front Matter
-
-
Modulation and monitoring of cell metabolism
About this book
Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Â
Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.
Editors and Affiliations
-
The Anlyan Center, Department of Genetics, Yale School of Medicine, New Haven, USA
Peter M. Rabinovich
Bibliographic Information
Book Title: Synthetic Messenger RNA and Cell Metabolism Modulation
Book Subtitle: Methods and Protocols
Editors: Peter M. Rabinovich
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-260-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-259-9Published: 08 January 2013
Softcover ISBN: 978-1-4939-5967-9Published: 23 August 2016
eBook ISBN: 978-1-62703-260-5Published: 09 January 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 323
Topics: Human Genetics