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  • Book
  • © 2012

DNA Repair Protocols

Editors:

  • Covers both mammalian and non-mammalian model organisms
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 920)

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Table of contents (40 protocols)

  1. Front Matter

    Pages i-xvii
  2. Identification of Repair Genes and Generation of Repair Mutants

    1. Front Matter

      Pages 7-7
    2. Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening

      • Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard, Anders Olsen
      Pages 27-38
    3. Establishment of the DNA Repair-Defective Mutants in DT40 Cells

      • Masamichi Ishiai, Emi Uchida, Minoru Takata
      Pages 39-49
  3. Assessing DNA Damage and Repair

    1. Front Matter

      Pages 51-51
    2. Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair

      • Chun Tsai, Vaughn Smider, Byung Joon Hwang, Gilbert Chu
      Pages 53-78
    3. Detection of DNA Damage by Comet Fluorescence In Situ Hybridization

      • Wiebke Schlörmann, Michael Glei
      Pages 91-100
    4. Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitative PCR

      • Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, Bennett Van Houten
      Pages 111-132
  4. Repair of Incorrect or Damaged DNA Bases

    1. Front Matter

      Pages 133-133
    2. In Vitro DNA Mismatch Repair in Human Cells

      • Liya Gu, Charles M. Ensor, Guo-Min Li
      Pages 135-147
    3. Eukaryotic DNA Mismatch Repair In Vitro

      • Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang
      Pages 149-162
    4. Immuno-Slot Blot Assay for Detection of UVR-Mediated DNA Damage

      • Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, Marcus S. Cooke
      Pages 163-175
    5. Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies

      • Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, Orlando D. Schärer
      Pages 203-219
    6. Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extracts

      • Puck Knipscheer, Markus Räschle, Orlando D. Schärer, Johannes C. Walter
      Pages 221-243

About this book

Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication.

Reviews

From the book reviews:

“This book covers important aspects of the eukaryotic response to genomic insults and the most recent and advanced protocols on DNA repair, recombination, and replication. … The intended audience is researchers in the field of DNA repair. In my judgment, graduate students, postdoctorals, and researchers in the field of molecular biology, especially those with a focus on DNA repair, will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, February, 2013)

Editors and Affiliations

  • , Department of Molecular Biology, Aarhus University, Århus C, Denmark

    Lotte Bjergbæk

Bibliographic Information

  • Book Title: DNA Repair Protocols

  • Editors: Lotte Bjergbæk

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-998-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2012

  • Hardcover ISBN: 978-1-61779-997-6Published: 01 September 2012

  • Softcover ISBN: 978-1-4939-5954-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-998-3Published: 23 October 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVII, 631

  • Topics: Human Genetics, Gene Function

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access