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Progenitor Cells

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Kimberly A. Mace

    1. Fac. Life Sciences, Healing Foundation Centre, University of Manchester, Manchester, United Kingdom

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  2. Kristin M. Braun

    1. School of Medicine & Dentistry, Centre for Cutaneous Research, Blizard I, Barts and The London, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementaion advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 916)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
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  2. In Vitro Culture Systems

    1. Front Matter

      Pages 1-1
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  3. In vitro Culture Systems

    1. Isolation of Satellite Cells from Single Muscle Fibers from Young, Aged, or Dystrophic Muscles

      • Valentina Di Foggia, Lesley Robson
      Pages 3-14

    2. Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates

      • John T. Connelly
      Pages 15-22

    3. Isolation, Culture, and Potentiality Assessment of Lung Alveolar Stem Cells

      • Feride Oeztuerk-Winder, Juan-Jose Ventura
      Pages 23-30

    4. Three-Dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells

      • Fatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, Paul G. Genever
      Pages 31-45

    5. Isolation of Adult Stem Cells and Their Differentiation to Schwann Cells

      • Cristina Mantovani, Giorgio Terenghi, Susan G. Shawcross
      Pages 47-57

    6. Functional Purification of Human and Mouse Mammary Stem Cells

      • Daniela Tosoni, Pier Paolo Di Fiore, Salvatore Pece
      Pages 59-79

    7. Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells

      • S. Bahram Bahrami, Mandana Veiseh, Nancy J. Boudreau
      Pages 81-96

  4. Nonmammalian Model Systems

    1. Front Matter

      Pages 97-97
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  5. Non-mammalian Model Systems

    1. Transcriptome Analysis of Drosophila Neural Stem Cells

      • Katrina S. Gold, Andrea H. Brand
      Pages 99-110

    2. Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo

      • Alexia Rabilotta, Rana Amini, Jean-Claude Labbé
      Pages 111-125

    3. Tol2-Mediated Gene Transfer and In Ovo Electroporation of the Otic Placode: A Powerful and Versatile Approach for Investigating Embryonic Development and Regeneration of the Chicken Inner Ear

      • Stephen Freeman, Elena Chrysostomou, Koichi Kawakami, Yoshiko Takahashi, Nicolas Daudet
      Pages 127-139

    4. Labeling Primitive Myeloid Progenitor Cells in Xenopus

      • Ricardo Costa, Yaoyao Chen, Roberto Paredes, Enrique Amaya
      Pages 141-155

    5. Identification of Oocyte Progenitor Cells in the Zebrafish Ovary

      • Bruce W. Draper
      Pages 157-165

    6. FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms

      • Belen Tejada Romero, Deborah J. Evans, A. Aziz Aboobaker
      Pages 167-179

    7. Clonal and Lineage Analysis of Melanocyte Stem Cells and Their Progeny in the Zebrafish

      • Robert C. Tryon, Stephen L. Johnson
      Pages 181-195

    8. Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted Neurospheres

      • Levan Mchedlishvili, Vladimir Mazurov, Elly M. Tanaka
      Pages 197-202

    9. Following the Fate of Neural Progenitors by Homotopic/Homochronic Grafts in Xenopus Embryos

      • Raphaël Thuret, Nancy Papalopulu
      Pages 203-215

  6. Mammalian Model Systems

    1. Front Matter

      Pages 217-217
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Keywords

About this book

Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types.  This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems.

Editors and Affiliations

  • Fac. Life Sciences, Healing Foundation Centre, University of Manchester, Manchester, United Kingdom

    Kimberly A. Mace

  • School of Medicine & Dentistry, Centre for Cutaneous Research, Blizard I, Barts and The London, London, United Kingdom

    Kristin M. Braun

Bibliographic Information

  • Book Title: Progenitor Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kimberly A. Mace, Kristin M. Braun

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-980-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-979-2Published: 23 August 2012

  • Softcover ISBN: 978-1-4939-5882-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-980-8Published: 23 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 408

  • Topics: Cell Biology, Stem Cells

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