Overview
- Provides step-by-step laboratory protocols, from conventional methods to state-of-the-art high throughput approaches and even computational data processing techniques
- Contains expert tips on troubleshooting known pitfalls
- Lists the necessary materials and provides the needed background information for all experiments
Part of the book series: Methods in Molecular Biology (MIMB, volume 883)
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Table of contents (17 protocols)
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Front Matter
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Back Matter
Keywords
About this book
RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.Editors and Affiliations
Bibliographic Information
Book Title: RNA Abundance Analysis
Book Subtitle: Methods and Protocols
Editors: Hailing Jin, Walter Gassmann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-839-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-838-2Published: 21 May 2012
Softcover ISBN: 978-1-4939-5916-7Published: 23 August 2016
eBook ISBN: 978-1-61779-839-9Published: 16 May 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 231
Number of Illustrations: 22 b/w illustrations
Topics: Human Genetics, Gene Expression