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  • © 2012

Microbial Systems Biology

Methods and Protocols

Editors:

  • Introduction to state-of-the-art technologies that are being used to study microbial systems biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 881)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Sample Preparation and Identification

    1. Front Matter

      Pages 1-1
  3. Sample Prepartion and Identification

    1. Pressure Cycling Technology in Systems Biology

      • Bradford S. Powell, Alexander V. Lazarev, Greta Carlson, Alexander R. Ivanov, David A. Rozak
      Pages 27-62
    2. Bacterial Identification and Subtyping Using DNA Microarray and DNA Sequencing

      • Sufian F. Al-Khaldi, Magdi M. Mossoba, Marc M. Allard, E. Kurt Lienau, Eric D. Brown
      Pages 73-95
  4. Experimental Genomic Analyses

    1. Front Matter

      Pages 97-97
  5. Protein and Lipid Analyses

    1. Front Matter

      Pages 157-157
    2. Microbial Proteomics Using Mass Spectrometry

      • Harry B. Hines
      Pages 159-186
  6. Metabolomic Analyses

    1. Front Matter

      Pages 213-213
    2. Mass Spectrometry-Based Microbial Metabolomics

      • Edward E. K. Baidoo, Peter I. Benke, Jay D. Keasling
      Pages 215-278
    3. Fast Sampling of the Cellular Metabolome

      • Walter M. Van Gulik, Andre B. Canelas, Hilal Taymaz-Nikerel, Rutger D. Douma, Lodewijk P. de Jonge, Joseph J. Heijnen
      Pages 279-306
  7. Analyses of Cellular Metabolism

    1. Front Matter

      Pages 307-307
    2. Metabolic Pathway Determination and Flux Analysis in Nonmodel Microorganisms Through 13C-Isotope Labeling

      • Xueyang Feng, Wei-Qin Zhuang, Peter Colletti, Yinjie J. Tang
      Pages 309-330
    3. Biolog Phenotype Microarrays

      • April Shea, Mark Wolcott, Simon Daefler, David A. Rozak
      Pages 331-373
    4. NanoSIP: NanoSIMS Applications for Microbial Biology

      • Jennifer Pett-Ridge, Peter K. Weber
      Pages 375-408
  8. Kinetic Modeling of Cellular Processes

    1. Front Matter

      Pages 409-409

About this book

 Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli.  Although more and more efforts are being directed toward examining systems biology in complex multi-cellular organisms, the bulk of system-level analyses conducted to date have focused on the biology of microbes.  In, Microbial Systems Biology: Methods and Protocols expert researchers in the field describe the utility and attributes of different tools (both experimental and computational) that are used for studying microbial systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microbial Systems Biology: Methods and Protocols introduces and aids scientists in using the various tools that are currently available for analysis, modification and utilization of microbial organisms.

Reviews

From the reviews:

“This book … deals with one of the most dynamic trends in Biology, namely Systems Biology of Microorganisms. … Most of the chapters are of direct and immediate usefulness for scientists to start their own experiments with respect to different aspects of systems biology of a given microorganism or community/environment. … In my opinion, the purpose of this book … to introduce the reader to some of the established and state-of-the-art technologies that are being used to study microbial systems biology – is fully accomplished.” (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)

Editors and Affiliations

  • Physics & Life Sciences Directorate, Biosciences & Biotechnology Div., Lawrence Livermore National Laboratory, Livermore, USA

    Ali Navid

Bibliographic Information

  • Book Title: Microbial Systems Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Ali Navid

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-827-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-826-9Published: 27 May 2012

  • Softcover ISBN: 978-1-4939-6192-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-827-6Published: 26 May 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 615

  • Number of Illustrations: 73 b/w illustrations, 81 illustrations in colour

  • Topics: Microbiology, Systems Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access