Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step details essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 872)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.
Reviews
From the reviews:
“Robert M. Hoffman introduces In vivo Cellular Imaging Using Fluorescent proteins, the eighteen chapters book dedicated to the description of how fluorescence proteins have changed the way to analyze cellular processes in vivo. … This book is very well structured with several colored figures … . this is a useful book not only for those who study the biology of different cancer cells, but also for those who are interested in following the in vivo dynamic of cellular processes.” (Manuela Monti, European Journal of Histochemistry, Vol. 56, 2012)Editors and Affiliations
Bibliographic Information
Book Title: In Vivo Cellular Imaging Using Fluorescent Proteins
Book Subtitle: Methods and Protocols
Editors: Robert M. Hoffman
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-797-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2012
Hardcover ISBN: 978-1-61779-796-5Published: 11 June 2012
Softcover ISBN: 978-1-4939-6149-8Published: 23 August 2016
eBook ISBN: 978-1-61779-797-2Published: 15 June 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 269
Number of Illustrations: 6 b/w illustrations, 41 illustrations in colour
Topics: Imaging / Radiology