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Planar Cell Polarity

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Kursad Turksen

    1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Gathers together timely protocols on its vital subject area
  • Features lab-ready methods that have been proven successful
  • Includes key tips and expert implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 839)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

    • Daryl J. V. David, Melanie A. McGill, R. F. Andrew McKinley, Tony J. C. Harris
    Pages 1-17

  3. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

    • Bertrand Jauffred, Yohanns Bellaiche
    Pages 19-25

  4. Protein–Protein Interaction Techniques: Dissect PCP Signaling in Xenopus

    • Yingqun Wang
    Pages 27-41

  5. Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity

    • David Neff, Justin Hogan, Simon Collier
    Pages 43-52

  6. Analysis of Cell Shape and Polarity During Zebrafish Gastrulation

    • Douglas C. Weiser, David Kimelman
    Pages 53-68

  7. Analyzing Planar Cell Polarity During Zebrafish Gastrulation

    • Jason R. Jessen
    Pages 69-78

  8. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopus Gastrulation

    • Gun-Hwa Kim, Edmond Changkyun Park, Jin-Kwan Han
    Pages 79-89

  9. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

    • Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, Ira O. Daar
    Pages 91-104

  10. Gene Loss-of-Function and Live Imaging in Chick Embryos

    • Anne C. Rios, Christophe Marcelle, Olivier Serralbo
    Pages 105-117

  11. Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation

    • Courtney Mezzacappa, Yuko Komiya, Raymond Habas
    Pages 119-131

  12. Convergent Extension Analysis in Mouse Whole Embryo Culture

    • Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D. E. Greene, Andrew J. Copp
    Pages 133-146

  13. Analysis of PCP Defects in Mammalian Eye Lens

    • Yuki Sugiyama, John W. McAvoy
    Pages 147-156

  14. Examining Planar Cell Polarity in the Mammalian Cochlea

    • Helen May-Simera, Matthew W. Kelley
    Pages 157-171

  15. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

    • Lisa Fujimura, Masahiko Hatano
    Pages 173-185

  16. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

    • Kristi LaMonica, Laura Grabel
    Pages 187-200

  17. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

    • Mitsuharu Endo, Michiru Nishita, Yasuhiro Minami
    Pages 201-214

  18. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

    • Shinji Matsumoto, Akira Kikuchi
    Pages 215-227

  19. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

    • Makoto Matsuyama, Akihiko Shimono
    Pages 229-237

  20. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

    • Daniel J. Jagger, Andrew Forge
    Pages 239-248
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Keywords

About this book

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.  In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world.  As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Planar Cell Polarity

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-510-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-509-1Published: 05 January 2012

  • Softcover ISBN: 978-1-4939-5906-8Published: 30 April 2017

  • eBook ISBN: 978-1-61779-510-7Published: 06 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 262

  • Number of Illustrations: 32 b/w illustrations, 27 illustrations in colour

  • Topics: Cell Biology, Cell Physiology

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