Editors:
- Presents model systems and state-of-the-art techniques developed and perfected by leading scientists in the field
- Includes step-by-step protocols that are ready for the lab
- Features expert tips and key implementation advice in order to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 798)
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Table of contents (33 protocols)
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Front Matter
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Isolation and Analysis of Skeletal Muscle Precursor Cells
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Front Matter
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Non-Mammalian Models of Myogenesis
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Front Matter
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Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse
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Front Matter
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Generation of Viral Vectors and Transgenic Mice
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Front Matter
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About this book
Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology. Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells. Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume. Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.Editors and Affiliations
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Chicago Medical School, Dept. Cell Biology & Anatomy, Rosalind Franklin Univ of Med & Sci, North Chicago, USA
Joseph X. DiMario
Bibliographic Information
Book Title: Myogenesis
Book Subtitle: Methods and Protocols
Editors: Joseph X. DiMario
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-343-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-342-4Published: 01 November 2011
Softcover ISBN: 978-1-4939-5890-0Published: 23 August 2016
eBook ISBN: 978-1-61779-343-1Published: 02 December 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 558
Number of Illustrations: 71 b/w illustrations, 15 illustrations in colour
Topics: Cell Biology